The inhibitor CEP-33779 is a specific selective inhibitor of JAK2. In most somatic cells, JAK2 plays essential roles in cellular signal transduction and in the regulation of cell cycle. Little is known regarding the effects of JAK2 on mammalian oocyte maturation. In the present study, we investigated the effects of CEP-33779 on mouse oocytes meiosis and possible mechanisms of JAK2 during mouse ...

The aim of this study was to investigate the effect of different cryoprotectants like glycerol and DMSO individually and in combination of both on morphology of sheep oocyte using vitrification. The vitrification of sheep oocyte was performed using 40% glycerol and 40% DMSO individually and in combination of 20% glycerol + 20% DMSO. It was found that combination of cryoprotectants proved more e...

The quality of the human oocyte determines the success of fertilization and affects the consequent embryo development, pregnancy and birth; it therefore serves as a basis for human reproduction and fertility. The possibility to evaluate oocyte quality in the in vitro fertilization programme is very limited. The only criterion which is commonly used to evaluate oocyte quality is its morphology. ...

background: Many variations in oocyte and embryo grading make inter-laboratory comparisons extremely difficult. This paper reports the proceedings of an international consensus meeting on oocyte and embryo morphology assessment. methods: Background presentations about current practice were given. results: Theexpert panel developed a set of consensus points to define theminimumcriteria foroocyte...

Caffeine, as an oocyte aging inhibitor, was used in many different species to control or delay oocyte aging. However, the safety of caffeine and developmental competence of aged oocytes inhibited by caffeine has not been studied systematically. So we detected the spindle morphology, distribution of cortical granules, zona pellucida hardening and pronucleus formation to assess oocyte quality of ...

BACKGROUND Abnormal oocyte morphology has been associated with the hormonal environment to which the gametes are exposed. OBJECTIVE In this study, we evaluated the oocytes morphology, fertilization rate, embryos quality, and implantation rate resulted of retrieved oocytes in different times after human chorionic gonadotrophin (HCG) administration. MATERIALS AND METHODS A total of 985 metaph...

Reproductive cessation is perhaps the earliest aging phenotype that humans experience. Similarly, reproduction of Caenorhabditis elegans ceases in mid-adulthood. Although somatic aging has been studied in both worms and humans, mechanisms regulating reproductive aging are not yet understood. Here, we show that TGF-β Sma/Mab and Insulin/IGF-1 signaling regulate C. elegans reproductive aging by m...