TG572 RFLP Probe: Five primers were designed around the RFLP probe on chromosome 7: P572F1, P572F2, P572F3, P572R1, and P572R2 (Table 1). All possible primer combinations produced a strong band with Heinz 1706 DNA between 600 bp and 1000 bp (Fig. 2). All of the primer combinations produced single bands with the exception of P572F2/P572R1, which produced two bands one at 400 bp and one at 900 bp...

Background: In plant breeding program to produce hybrid varieties, pair of male sterile and restorer fertility lines are required. Differentiation of lines possessing restorer fertility allele from the lines lacking it remove the need for the progeny test, and thus reducing the time and the cost in the hybrid production procedure. Canola breeding program in Iran has concentrate...

all species in the genus lysiphlebus förster (hymenoptera: braconidae, aphidiinae) are solitary endoparasitoids of aphids, which are one of the most difficult and unknown taxonomic groups to identify. a pair of species-specific primers as a molecular marker was designed to identify the most abundant and important aphid parasitoid wasp, l. fabarum marshall, collected from guilan province (iran)....

Fingerprinting with molecular markers allows precise, objective and rapid variety identification. In this study, simple sequence repeat (SSR) markers were used to fingerprint the forty-eight main commercial rice cultivars under cultivation at present in Zhejiang Province, China, and identify indica and japonica subspecies including eight groups of closely related cultivars. Eighteen of the thir...

By comparing nucleic acid sequences determined for one of the most variable areas of 23S rRNA genes of 23 Campylobacter strains, we were able to identify regions specific for thermophilic Campylobacter strains. Oligonucleotide primers corresponding to these unique regions were synthesized and used in the polymerase chain reaction. One primer pair selectively detected all thermophilic Campylobac...

The efficacy of random primer-pair arrays compared to conventional RAPD method with a single decamer primer was evaluated using DNA from two species of Cucumis. The banding patterns of amplicons revealed enhanced utility of primer-pair arrays over conventional RAPDs, producing more bands and a higher degree of polymorphism, both at intra- and inter-specific levels. Amplification produced by bot...

To the Editor: The diagnoses of these cases were based on cellulose acetate electrophoresis, isoelectric focusing, or direct sequencing of the entire globin genes. For the detection of Hb CS, two pairs of primers were used by alteration of the structure of one of the globin chains of the hefor PCR. The first pair of primers (upstream primer, 5’moglobin (Hb) molecule.’ The diagnosis of these dis...

Brucellosis is a widespread zoonosis. Currently the diagnosis of this zoonosis is based on microbiological and serological laboratory tests. Polymerase chain reaction (PCR) has been used to detect DNA from Brucella. Different target genes, primer pairs, PCR techniques, and extraction procedures have previously been published for Brucella detection. But only a few of these primers have been used...

The fastidious bacterium causing citrus greening disease occurs in uneven and low concentrations in the sieve tubes of host plants[ A rapid and sensitive assay based on the polymerase chain reaction "PCR# has been developed using the primers derived from the sequences of the cloned DNA fragment of greening fastidious bacterium "GFB# to detect GFB infection in citrus[ One set of the primer pairs...