High-density oligonucleotide arrays can be used to rapidly examine large amounts of DNA sequence in a high throughput manner. An array designed to determine the specific nucleotide sequence of 705 bp of the rpoB gene of Mycobacterium tuberculosis accurately detected rifampin resistance associated with mutations of 44 clinical isolates of M. tuberculosis. The nucleotide sequence diversity in 121...

A method for analyzing combinatorial DNA arrays using oligonucleotide-modified gold nanoparticle probes and a conventional flatbed scanner is described here. Labeling oligonucleotide targets with nanoparticle rather than fluorophore probes substantially alters the melting profiles of the targets from an array substrate. This difference permits the discrimination of an oligonucleotide sequence f...

BACKGROUND Molecular barcode arrays provide a powerful means to analyze cellular phenotypes in parallel through detection of short (20-60 base) unique sequence tags, or "barcodes", associated with each strain or clone in a collection. However, costs of current methods for microarray construction, whether by in situ oligonucleotide synthesis or ex situ coupling of modified oligonucleotides to th...

1. Abstract 2. Introduction 3. Material and Methods 3.1. Animals 3.2. PCR Genotyping 3.3. Plasma determinations 3.4. Blood pressure 3.5. Histological analysis 3.6. RNA isolation, Affymetrix oligonucleotide array hybridization, and data analysis 3.7. Statistical analysis 4. Results 4.1. Plasma biochemistry 4.2. Arterial blood pressure 4.3. Plasma NO levels 4.4. Histological analyses of aorta 4.5...

AIM A rapid analysis of mitochondrial DNA (mtDNA) sequences with an array of immobilized sequence-specific oligonucleotide (SSO) probes was tested on 18 skeletal elements recovered from mass graves in Croatia, which could not be genotyped with common forensic nuclear DNA systems (PM+DQA1 and short tandem repeat analysis). METHODS We used duplex polymerase chain reaction (PCR) amplification of...

In this paper, a simple, reliable and flexible method, which integrated in situ synthesis with the spotting technique, was reported to fabricate oligonucleotide array. Different oligonucleotide sequences are synthesized on their relative code glass slides through combinational chemistry, thus the slides are broken into smaller pieces, in which the same code pieces have the same probe sequences....

Gene Identifier v.s. Oligonucleotide Identifier Oligonucleotides (often simply referred to as oligos or oligomers), which are typically between 25 to 75 bases long, have been extensively used by DNA microarray manufacturers to detect the expression of different genes. An oligonucleotide sequence that is located on a specific bead or position on a microarray is usually referred to as the probe s...

To facilitate sequence-specific detection of DNA amplified in a diagnostic reverse transcription (RT)-PCR for Lassa virus, we developed an array featuring 47 oligonucleotide probes for post-PCR hybridization of the amplicons. The array procedure may be performed with low-tech equipment and does not take longer than agarose gel detection.