Genomic in situ hybridization (GISH) has been widely used to detect rye (Secale cereale L.) chromosomes in wheat (Triticum aestivum L.) introgression lines. The routine procedure of GISH using genomic DNA of rye as a probe is time-consuming and labor-intensive because of the preparation and labeling of genomic DNA of rye and denaturing of chromosomes and probes. In this study, new oligonucleoti...

BACKGROUND Chromosomal rearrangements induced by wheat-rye hybridization is a very well investigated research topic. However, the structural alterations of wheat chromosomes in wheat-rye hybrids are seldom reported. METHODOLOGY/PRINCIPAL FINDINGS Octoploid triticale lines were derived from common wheat Triticum. aestivum L. 'Mianyang11'×rye Secale cereale L. 'Kustro'. Some progeny were obtain...

Fluorescein-labeled oligodeoxynucleotides (oligos) were introduced into cultured rat myoblasts, and their molecular movements inside the nucleus were studied by fluorescence correlation spectroscopy (FCS) and fluorescence recovery after photobleaching (FRAP). FCS revealed that a large fraction of both intranuclear oligo(dT) (43%) and oligo(dA) (77%) moves rapidly with a diffusion coefficient of...

Several studies have reported the efficacy of local therapies for lung cancer patients with postoperative oligo-recurrence. However, preference ablative over specific tyrosine-kinase inhibitors oligo-recurrence driver mutations remains controversial. We aimed to investigate optimal initial treatment strategy in mutations. Among 2,159 adenocarcinoma who underwent surgical resection at our instit...

The formation of reovirus double-stranded (ds) RNA and of oligo adenylic acid (oligo A) is inhibited by 5 mug of actinomycin D per ml added at the time of viral infection. Viral proteins are synthesized and assembled into dsRNA-deficient particles under these conditions. The addition of cycloheximide to infected cells during the mid-logarithmic phase of viral replication terminates protein and ...

the qom formation, which hosts the mazraeh celestite deposit, contains dominantly limestones, marl and subordinate evaporites deposited in shallow marine environments. petrographic evidence indicates that the celestite deposits were formed by replacement of limestone. the presence of carbonate inclusions within celestite crystals as well as the pseudomorphic habits of some crystals suggests tha...

SOCIO and 52H10 are two DNA clones previously reported by us to be over-expressed in human colonie mucosa at high risk for development of colonie tumors. This report presents sequencing data that reveal that these clones contain repetitive AIu elements. Each Alu sequence is associated with a 3'-oligoadenylate [oligo(A)] sequence, which is demonstrated to exhibit instability in human colonie tum...

PURPOSE To evaluate the outcomes of stereotactic body radiation therapy (SBRT) for patients with liver oligo-recurrence and oligo-progression from various primary tumors. MATERIALS AND METHODS Between 2002 and 2013, 72 patients with liver oligo-recurrence (oligo-metastasis with a controlled primary tumor) and oligo-progression (contradictory progression of a few sites of disease despite an ov...

Systematic secondary structure simulation of a target mRNA sequence is shown to be effective for locating a good anti-sense target site. Multiple selected anti-sense sequences were placed in a single molecule. The anti-sense oligonucleotide (oligo) was covalently closed to avoid exonuclease activities and was designated CMAS (covalently closed multiple anti-sense)-oligo. CMAS-oligo was found to...