The potential use of different hydrophobic substrates (oleic acid, oleyl alcohol, methyl oleate and rapeseed oil) for the production high concentrations 9-octadecenedioic acid by genetically engineered C. tropicalis ATCC-20962 yeast was researched. For comparison purposes, data growth, intracellular triacylglyceride (TAG) accumulation dicarboxylic (DCA) were obtained mathematical description us...

Wax esters are neutral lipids exhibiting desirable properties for lubrication. Natural sources have traditionally been whales. Additionally some plants produce wax esters in their seed oil. Currently there is no biological source available for long chain length monounsaturated wax esters that are most suited for industrial applications. This study aimed to identify enzymatic requirements enabli...

Diacylglycerols composed of one saturated and one unsaturated chain make up the hydrophobic core of many biological membranes. We report here the first crystalline structure of such a mixed chain diacylglycerol. The mixed chain diacylglycerol, 1-stearoyl-3-oleyl-glycerol ( 1,3-SODG) was produced by solution isomerization of 1-stearoyl-2-oleylsn-glycerol. 1.3-SODG was isolated by flash chromatog...

The miscibility of 1-palmitoyl-2-oleoyl phosphatidylcholine with triolein, 1,2-diolein, 1,3-diolein, 1(3)-monoolein, oleyl alcohol, methyl oleate, oleic acid, and oleyl cyanide (18:1 lipids) was studied at the argon-water interface. The isothermal phase diagrams for the mixtures at 24 degrees were characterized by two compositional regions. At the limit of miscibility with lower mol fractions o...

Objective(s): Oleate can be produced through de novo synthesis, which contributes to biological processes and signaling pathways. However, the role of this non-essential fatty acid in hepatic development remains unclear. The current study aimed to evaluate the influence of early oleate deficiency induced by the inhibitor of de novo oleate synthesis MF-438 on fetal rat ...

Oxysterol binding protein (OSBP) related proteins (ORPs) constitute a family that has at least 12 members in humans. In the present study we characterize one of the novel OSBP homologs, ORP2, which we show to be expressed ubiquitously in mammalian tissues. The ORP2 cDNA encodes a deduced 55 kDa protein that lacks a pleckstrin homology (PH) domain, a feature found in the other family members. Su...

Various organic compounds used as diluents, modifiers and extractants in lactic acid extraction have been tested to determine their toxicity towards Lactobacillus casei cells. The toxicity on molecular and phase level has been investigated. In general, the tested hydrocarbons were non-toxic on molecular level and showed variable toxicity on phase level. Among the tested alcohols octanol and ole...

There are specific phorbol ester receptors on cardiac myocytes which may be identical with the calcium/phospholipid-dependent protein kinase (protein kinase C). Incubation of enzymatically dissociated rat cardiac myocytes with biologically active phorbol esters (such as 4 beta-phorbol-12, 13-dibutyrate and 12-O-tetradecanoyl phorbol-13-acetate) leads to a time- and concentration-dependent loss ...