نتایج جستجو برای: of which 17 primers produced polymorphic bands these 17 selected rapd primers produced 233 bands

تعداد نتایج: 21252121  

M. Sadehghizadeh, M.R. Kalbasi, S. Jamshidi,

Genetic variation and inheritance of randomly amplified polymorphic DNA (RAPD) markers in female grass carp (Ctenopharyngodon idella) and male bighead carp (Hypophthalmichthys nobilis) and their F1 offspring hybrid have been studied. For this purpose, genomic DNA was extracted from muscle tissue according to phenol-chloroform method, and six decamer primers were used for amplifying polymorphic ...

M. Sadehghizadeh M.R. Kalbasi, S. Jamshidi,

Genetic variation and inheritance of randomly amplified polymorphic DNA (RAPD) markers in female grass carp (Ctenopharyngodon idella) and male bighead carp (Hypophthalmichthys nobilis) and their F1 offspring hybrid have been studied. For this purpose, genomic DNA was extracted from muscle tissue according to phenol-chloroform method, and six decamer primers were used for amplifying polymorphic ...

2014
Nawroz Abdul-razzak Tahir

Genetic variability and relationships among ten rice varieties were studied by using 20 decamer random primers and SDSPAGE methods. Out of 22, 20 Random Amplified Polymorphic DNA (RAPD) primers revealed polymorphism, while the remaining 2 primers showed no reaction. The primers produced a total of 109 bands, of which 62 (56.88%) were polymorphic. The number of polymorphic fragments for each pri...

Genetic diversity of 12 lemon balm ecotypes was evaluated using agronomic traits as well as ISSR and RAPD markers. Results of analysis of variance showed high diversity among the studied ecotypes. Cluster analysis using UPGMA method grouped the ecotypes into three different groups based on the agronomic traits. Twelve ISSR primers created 106 polymorphic bands among the studied ecotypes. An ISS...

M. R. Kalbassi, S. Jamshidi ,

  Random amplified polymorphic DNA (RAPD) markers were used to estimate genetic relationships between two seasonal immigrant forms (namely fall-run and spring-run) of Caspian trout Salmo trutta caspius. In this regard, 62 fin tissue samples were collected from southern parts of the Caspian Sea basin and examined with 16 oligodecamer primers to asses their genetic diversity as well as probable s...

  Random amplified polymorphic DNA (RAPD) markers were used to estimate genetic relationships between two seasonal immigrant forms (namely fall-run and spring-run) of Caspian trout Salmo trutta caspius. In this regard, 62 fin tissue samples were collected from southern parts of the Caspian Sea basin and examined with 16 oligodecamer primers to asses their genetic diversity as well as probable s...

Journal: :journal of agricultural science and technology 2014
p. sharma v. sharma v. kumar

in the present study, random amplified polymorphic dna (rapd) and inter-simple sequence repeat (issr) markers were used to estimate genetic diversity and relationships among 35 cluster bean [cyamopsis tetragonoloba (l.) taub] genotypes. for rapd analysis, 20 random primers were used which amplified 164 bands, 147 of which were polymorphic with an average polymorphism of 90.3%. the amplified pro...

Journal: :پژوهش های حبوبات ایران 0
سمانه ذکائی ماهرخ فلاحتی رستگار بهروز جعفرپور عبدالرضا باقری وحید جهانبخش مشهدی

in order to study the genetic diversity of the causal agent of fusarium wilting in chickpea, two techniques including rapd and pcr-rflp were used. in rapd-pcr technique, all 14 primers could show the diversity among the isolates. after studding the banding patterns of 14 primers, a dendrogram based on similarity matrix was constructed. the rapd dendrogram analysis could not separate isolates ba...

2012
Niraj Tripathi Devendra Singh Chouhan Navinder Saini Sharad Tiwari

Genetic variations of 15 Brahmi (Bacopa monnieri L.) accessions were evaluated using random amplified polymorphic DNA (RAPD) and inter simple sequence repeats (ISSR) markers. During RAPD analysis, amplification of genomic DNA of the 15 accessions by 22 primers generated 197 fragments, of which 187 were polymorphic with an average of 8.95 bands per primer. The amplified products varied in size f...

Journal: :Genetics and molecular research : GMR 2011
A I Khan I A Khan F S Awan H A Sadaqat S Bahadur

The genetic distance of 11 cotton genotypes varying in heat tolerance was studied using RAPD markers. Fifty-three random decamer primers were used for the estimation of genetic distance. Among the 53 RAPD primers, which were custom synthesized by GeneLink Inc., UK, 32 were polymorphic and 21 were monomorphic. The 32 polymorphic primers produced 273 fragments, with a mean of 8.3 fragments per pr...

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