Surfactants represent one of the most common constituents in topical pharmaceutical and cosmetic applications or cleansers. Since adverse skin and ocular reactions can be caused by them, it is important to evaluate damaging effects. Amino acid-based surfactants deserve particular attention because of their low toxicity and environmental friendly properties. New lysine derivative surfactants ass...

Two optimized pranoprofen-loaded poly-l-lactic-co glycolic acid (PLGA) nanoparticles (PF-F1NPs; PF-F2NPs) have been developed and further dispersed into hydrogels for the production of semi-solid formulations intended for ocular administration. The optimized PF-NP suspensions were dispersed in freshly prepared carbomer hydrogels (HG_PF-F1NPs and HG_PF-F2NPs) or in hydrogels containing 1% azone ...

The main object of our study was to investigate whether the resazurin metabolism assay is a sensitive surfactant and alcohol toxicity test in isolated pig cornea and to compare this recently developed fluorometric assay with the data collected in the eye irritation reference chemical data bank. Resazurin is a substrate that changes color in response to metabolic activity. Isolated pig corneas w...

Currently, the European Centre for the Validation of Alternative Methods (ECVAM) has supported formal validation studies on in vitro tests for predicting skin irritancy and corrosivity, which included three of the in vitro tests employed human skin models, EPISKINTM and EpiDermTM. When skin models are used to evaluate skin irritancy and corrosivity, it is important that suitable chemical applic...

Natamycin, an antifungal agent that has been approved in the management of exteriorfungal infectionsof oculus such asfungal keratitis,blepharitis, and conjunctivitis. The ophthalmic preparation needs frequent installation into eye due to quickprecorneldrugloss which may lead poor bioavailability. present investigation aimed at formulation development characterization situophthalmic gel natamyci...

To determine whether aqueous and ethanol fractions of the Angelica keiskei leaf exert toxicity when used for cosmetic purposes, we performed the acute eye irritancy test. Animals were treated with sample fractions (100 mg/dose) according to standard procedure guidelines. No significant changes or damage was detected in the fraction-treated groups in terms of ocular lesions in the cornea, the si...