A method has been developed for growing Physarum polycephalum plasmodia that are 8 to 10 times larger than those obtained in the petri dish cultures used by Nygaard, Guttes, and Rusch. In the large-scale procedure, plasmodia were grown in metal trays on a membrane supported by filter paper on stainless-steel screen. Plasmodia were started from a ring of inoculum to allow inward and outward migr...

The purpose of this study was to produce a novel pH sensitive hydrogel with superior thermal stability, composed of poly(acrylic acid) (PAA) and cellulose nanocrystal (CNC). CNC was extracted from kenaf fiber through a series of alkali and bleaching treatments followed by acid hydrolysis. PAA was then subjected to chemical cross-linking using the crosslinking agent (N,N-methylenebisacrylamide) ...

Rhizosphere investigations are restricted by the sheer number of cultures that must be handled in the multitude of different tubed media needed to characterize each isolate. A current rhizosphere study called for the use of 25 different diagnostic media with each of hundreds of isolates, a burdensome and laborious problem. Moreover, the replica plating technique of J. Lederberg and E. M. Lederb...

The cytotoxic effect of spermidine was investigated on various tissue culture cell lines in the presence of calf serum. Cells incubated with cytotoxic concentrations remained rounded up while cells in control cultures always spread out on the surface of the Petri dish. The spermidine concentration tolerated depends on cell number, concentration of serum, the strain of cells used and the phase o...

BACKGROUND Immunological response of host and parasite play a key role in developing vaccination and immunization. The present study deals with the immune response and effecter mechanism, which was confirmed by migration inhibition factor (MIF). METHODS The present work was conducted in Parasitological Lab of Postgraduate Department of Zoology, Government Holkar Science College, Indore (M.P.)...