Antimycin A (AMA) inhibits mitochondrial electron transport between cytochrome b and c. We recently demonstrated that AMA inhibits the growth of lung cancer Calu-6 cells and the changes of reactive oxygen species (ROS) and glutathione (GSH) levels affect apoptosis in Calu-6 cells. Here, we examined the effects of N-acetyl-cysteine (NAC, a well known antioxidant), L-buthionine sulfoximine (BSO, ...

since nox family, one of the most important causes of air pollution, is the primary source of acid rains, ozone layer depletion, and breathing disorders, mitigation of these pollutants is now a global concern. in the off-gases of internal combustion engines running with oxygen excess, non-thermal plasmas (ntps) have an oxidative potential, which results in an effective conversion of no to no2. ...

Although the use of modulator treatments targeting basal defect in Cystic Fibrosis transmembrane conductance regulator improves lung function and is associated with reduced burden infections, we have not seen bacterial eradication, thus antibiotic remain crucial for chronic Pseudomonas aeruginosa infection. Recent investigations shown large zones oxygen (O2) depletion infected part CF lungs due...

Arsenic trioxide (ATO; As2O3) induces cell death in various types of cancer cells including lung cancer via increasing reactive oxygen species (ROS) and regulating mitogen-activated protein kinase (MAPK) signaling cascades. However, little is known about the relationship between ATO and MAPK signaling in normal lung cells. Here, we investigated th...

Regional myocardial oxygenation was assessed during partial and complete coronary artery occlusion using near infrared spectroscopy. In eight open-chest dogs, partial occlusions resulting in an approximately 42% decrease in left anterior descending coronary artery (LAD) blood flow produced an approximately 21% decrease in tissue O2 stores (tissue oxyhemoglobin plus oxymyoglobin) and no change i...

Superoxide (O2(·-)) production by the NADPH oxidases is implicated in the pathogenesis of many cardiovascular diseases, including hypertension. We have previously shown that activation of NADPH oxidases increases mitochondrial O2(·-) which is inhibited by the ATP-sensitive K(+) channel (mitoKATP) inhibitor 5-hydroxydecanoic acid and that scavenging of mitochondrial or cytoplasmic O2(·-) inhibit...

Studies of rates of consumption of dissolved O2 by suspensions of bacteroids (Rhizobium japonicum, strain CB1809) from soybean root nodules showed the presence of two different terminal oxidase systems. A high-affinity system, sensitive to inhibition by N-phenylimidazole and by carbon monoxide, was most active when the dissolved O2 was between 0-01 and 0-1 muM. At 1 muM-O2 or higher, this oxida...

To determine whether glucose depletion is a principal determinant of hyperoxic cell death in vitro, human lung epithelial-like cells (A549) were exposed to hyperoxia (95% O2) in either 10, 30, or 50 ml of medium (Ham's F-12K). Glucose was depleted in the medium after 36, 60, or 96 h, respectively. Medium lactate dehydrogenase (LDH) activity increased only after glucose was depleted. To confirm ...

Agriculture is severely impacted by water stress due either to excess (hypoxia/anoxia) or deficit of water availability. Hypoxia/anoxia is associated with oxygen (O2) deficiency or depletion, inducing several anatomical, morphological, physiological, and molecular changes. The majority of these alterations are adaptive mechanisms to cope with low O2 availability; among them, alterations in shoo...

a significant reduction in arterial blood oxygen saturation during fiberoptic bronchoscopy has been proved but it is not yet known whether all patients need supplemental oxygen during this procedure. the aim of study is to examine the relationship between peak expiratory flow rate (pefr) before bronchoscopy and oxygen desaturation during bronchoscopy. measurement of pefr (% predicted) performed...