Protein Kinase A (PKA) is a macromolecular assembly composed of catalytic subunit (PKA-C) and regulatory (PKA-R) that harbors cyclic-nucleotide binding (CNB) domains. During its phosphorylation cycle, PKA-C experiences different conformational states depend on the nucleotides, metal ions substrates to active site. How changes in allosterically modulate interactions with CNB domains PKA-R curren...

MicroRNAs (miRNAs) are non-coding RNAs consisting of a length roughly 22 nucleotides that participate in gene regulation [...]

Using vegetable protein sources as a replacement for fish meal (FM) in the diet of Pacific white shrimp (PWS) has negative impact on their health. Acute hepatopancreatic necrosis disease (AHPND), caused by Vibrio parahaemolyticus, affects PWS and causes financial losses. Nucleotides modulate immune response could contribute to counteracting these issues. Our objective was evaluate effects nucle...

interferon regulatory factor 7 (irf7) gene was cloned from a subtractive cdna library constructed with mrnas obtained from rainbow trout (oncorhynchus mykiss) macrophage cell line (rts-11). using expressed sequence tag clones of submitted irf7 amino acid sequences, specific primers were designed. results showed that irf7 cdna contains an orf of 1251 nucleotides that translates into a 416 residu...

the interaction of magnesium hydrate at the phosphate oxygen atom of the pyrimidine nucleotides (cmp,ump,dtmp) were studied at the hartree-fock level theory. we used lanl2dz basis set for mg and 6-31g* basis set for atoms.the basis set superposition error (bsse) begins to converge for used method/basis set. the gauge-invariant atomic orbital (giao) method and the continuous-set-of-gauge-transfo...

DNA-based storage is considered to be a promising option accommodate huge amounts of data. The strings nucleotides are prone errors though. To reduce the error probability, these should satisfy constraints on ratio A's and T's versus number G's C's, maximum repeated identical nucleotides. deal with when they occur after all, it also desirable that set DNA-strings possesses certain correction or...

Diabetes mellitus causes changes in metabolism of extracellular nucleotides acting through P2 receptors (P2Rs). This affects renal function and may lead to glomerular tubular disturbances. We measured urinary excretion (ATP, ADP, AMP, UTP, UDP, UMP) streptozotocin-induced diabetic rats (65 mg/kg, i.p., day 0) the effects P2Rs’ blockade by suramin (10 days +7, +14) on P2×7R expression (albumin, ...