Unnatural nucleosides have been explored to expand the properties and the applications of oligonucleotides. This paper briefly summarizes nucleic acid analogs in which the base is modified or replaced by an unnatural stacking group for the study of nucleic acid interactions. We also describe the nucleoside analogs of a base pair-mimic structure that we have examined. Although the base pair-mimi...

A cascade CRISPR/cas nucleic acid diagnostic system, which can achieve high-sensitive and single-base specificity without target amplification, was developed for miRNA detection.

Nucleic acid detection in hematological disorders has been carried out in a wide range of medical fields or departments. Hence, it is recommended that the hematology laboratory should draw up guidelines or a manual for each disease (group), field or specialty. Nucleic acid detection is defined as processes which are disclosed using depolymerization of a nucleic acid hybrid (deoxyribonucleic aci...

An artificial nucleic acid analogue capable of self-assembly into duplex merely through hydrophobic interactions is presented. The replacement of Watson-Crick hydrogen bonding with strictly hydrophobic interactions has the potential to confer new properties and facilitate the construction of complex DNA nanodevices. To study how the hydrophobic effect works during the self-assembly of nucleic a...

Abstract Artificial metal base pairs have become increasingly important in nucleic acids chemistry due to their high thermal stability, water solubility, orthogonality natural pairs, and low cost of production. These interesting properties combined with ease chemical enzymatic synthesis prompted use several practical applications, including the construction nanomolecular devices, ions sensors, ...

background nucleic acid based detection of mycobacterium tuberculosis has the potential to improve the analysis of the tuberculosis epidemiology and patient care by increasing the specificity and sensitivity of diagnosis. patients and methods fifty suspected cases were screened for m. tuberculosis by using a new primer sequence and comparing its results with the primer (is6110) most widely used...

A new set of DNA base-nucleic acid codes and their hypercomplex number representation have been introduced for taking the probability of each nucleotide into full account. A new scoring system has been proposed to suit the hypercomplex number representation of the DNA base-nucleic acid codes and incorporated with the method of dot matrix analysis and various algorithms of sequence alignment. Th...