The Sindbis-group alphavirus S.A.AR86 encodes a threonine at nonstructural protein 1 (nsP1) 538 that is associated with neurovirulence in adult mice. Mutation of the nsP1 538 Thr to the consensus Ile found in nonneurovirulent Sindbis-group alphaviruses attenuates S.A.AR86 for adult mouse neurovirulence, while introduction of Thr at position 538 in a nonneurovirulent Sindbis virus background con...

Sindbis virus and Ross River virus are alphaviruses whose nonstructural proteins share 64% identity and whose structural proteins share 48% identity. Starting from full-length cDNA clones of both viruses, we have generated two reciprocal Sindbis-Ross River chimeric viruses in which the structural and nonstructural regions have been exchanged. These chimeric viruses replicate readily in several ...

article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Plasmids were constructed which contained a large portion of each of the four nonstructural genes of Sindbis virus fused to the N-terminal two-thirds of the trpE gene of Escherichia coli. The large quantity of fusion protein induced from cells containing these plasmids was subsequently used as an antigen to generate polyclonal antisera in rabbits. Each antiserum was specific for the correspondi...

background bovine viral diarrhea (bvd) is an economically important disease of cattle with a worldwide distribution. diagnosis of bvd relies on laboratory-based detections of its viral causing agent or virus specific antibodies. the most common laboratory method used for this purpose is the elisa. bovine viral diarrhea virus (bvdv) nonstructural protein 3 (ns3) is one of the most highly conserv...

GI disorders affect the quality of children. Children with Down syndrome have a high prevalence disorders. This study aims to determine life children is cross-sectional using PedsQL Symptoms Scale questionnaire & ROME IV Diagnostic Questionnaire for Pediatric Functional Gastrointestinal Disorder Adolescents. Samples were collected in January-May 2022. The analysis was performed IBM SPSS Sta...

objective(s): the rotavirus nonstructural protein 4 (nsp4) is responsible for the increase in cytoplasmic calcium concentration through a phospholipase c-dependent and phospholipase c-independent pathways in infected cells. it is shown that increasing of intracellular calcium concentration in rotavirus infected cells is associated with the activation of some members of protein kinases family su...

an application of aerated concret (foam concrete) was experimentally studied. in this study the compressive strength of the proposed concrete was measured the effect of parameters such as aggregate size of broken stone, quantity of cement and various densities were exprementally studies, and the best mixture was select.these prepared mixtures are practically useful to optimize the consumption o...

background: the h9n2 subtype of avian influenzaviruses (aivs) have spread in asia and middle east countriesand have become a serious threat to poultry industry in iran.objectives:characterization of genes of h9n2 subtype involvingin pathogenicity and diagnosis are crucial in control of avianinfluenza outbreaks. the nonstructural (ns) gene and its proteinproducts (ns1 & ns2) are important as...