the colon, adenocarcinoma of the breast) and on nonneoplastic tissues from cancer patients (liver, kidney, spleen, brain, and red blood cells). Eighteen distinct nonspecific esterase bands have been identified and characterized with respect to substrate preference and inhibitor profile. Each of the normal tissues contains a readily recognizable esterase pattern; the tumor extracts present great...

Virulent Nocardia asteroides reduces lysosomal acid phosphatase activity in murine macrophages. A computer-assisted imaging photometry system was used to quantitate lysozyme and nonspecific esterase-neutral protease levels within individual macrophages following ingestion of nocardiae. In contrast to acid phosphatase, lysozyme and esterase-neutral protease activity was either unchanged or incre...

TUDIES on the inheritance of nonspecific esterase forms of the house mouse (Mus musculus) have recently been reported from the serum (POPP and POPP 1962; PETRAS 1963), kidney ( RUDDLE and RODERICK 1965), and erythrocytes (PELZER 1965). A brief report (SHAW 1964) indicated inheritable esterase differences in kidney homogenates of the deer mouse, Peromyscus maniculatus, and two rare genetically d...

The formed elements of human blood each contain multipIe isoenzymes of nonspecific esterase that hydrolyze short chain alpha naphthyl esters. Zymograms that are characteristic of each type of formed element are obtained by subjecting purified preparations of each to polyacrylamide slab gel electrophoresis at pH 9.5 and subsequent staining of the gels for esterase activity. The most prominent is...

Nonspecific esterase (NSE) is used to identify normal and leukemic mononuclear phagocytes cytochemically. It can be demonstrated by the hydrolysis of alpha-naphthyl acetate or butyrate. NSE from leukapheresed leukemic monocytes were extracted with several types of detergent and grouped into two isoelectric point (pl) categories based on the ease of solubility: pl 5.7-6.3 (8 bands) greater than ...

HE existence in natural populations of a protein polymorphism involving Ttwo forms of a nonspecific esterase has been established in Drosophila mlamgaster (WRIGHT 1961, 1963). The two forms of the esterase, Esterase 6, are distinguishable by their different electrophoretic mobilities in starch gel, Esterase 6" migrating more rapidly toward the anode than Esterase 6s. The inheritance of these tw...