Functionally hepatectomized rabbits showed convulsions and coma within 2 hours after the intravenous injection of fat emulsion and heparin. Intravenous injection of the detergent triton prevented this syndrome, if given before the fat emulsion and heparin, and reversed it if given subsequently. Extremely high plasma non-esterified fatty acid levels were noted in rabbits showing the convulsive s...

Although many studies have been conducted on the components present in human breast milk (HM), research on the differences of chemical metabolites between HM, bovine milk (BM) and formula milk (FM) is limited. This study was to explore the chemical diversity of HM, BM and FM by metabolomic approaches. GC-TOFMS and UPLC-QTOFMS were applied to investigate the metabolic compositions in 30 HM sampl...

1. Experimental elevation of plasma non-esterified fatty acid concentrations has been postulated to decrease insulin-stimulated glucose oxidation and storage rates. Possible mechanisms were examined by measuring skeletal muscle glycogen synthase activity and muscle glycogen content before and during hyperinsulinaemia while fasting plasma non-esterified fatty acid levels were maintained. 2. Fast...

ANALYSIS OF NON-ESTERIFIED FATTY ACIDS IN HUMAN PLASMA BY CAPILLARY GAS-CHROMATOGRAPHY WITH SPLITLESS INJECTION. The aim of the present work was to test the combination of non-esterified fatty acid (NEFA) isolation using fumed silicon dioxide with capillary gas-chromatography (C-GC) with splitless injection for the analysis of NEFAs in human plasma. Injection volume, solvent re-condensation and...

Three major regulatory steps can be recognized in the control of ketogenesis (Fig. 1): (1) regulation of the supply of the major ketogenic substrate, plasma non-esterified fatty acids, by control of their release from adipose tissue; (2) regulation in the liver of the relative fluxes of non-esterified fatty acids through the pathways of /3-oxidation and esterification; (3) regulation in the liv...

The effects of inclusion of different fatty acids in the medium on the rate of esterification of palmitic acid and its stereospecific distribution among the three positions of the triacyl-sn-glycerols by preparations of rat adipocytes in vitro have been determined. Myristic acid, stearic acid, oleic acid and linoleic acid were used as diluents and the concentration of the combined unesterified ...

The complex lipids of the naturally-occurring general-fatty-acid-auxotroph Butyrivibrio S2 [Hazlewood & Dawson (1979) J. Gen. Microbiol. 112, 15-27] grown with palmitic acid as sole fatty-acid supplement have been investigated and some have been isolated in a state of purity and analysed. The majority are phospholipids (84%) and many contain galactose. They typically possess few esterified long...

Impaired fatty acid metabolism has been implied in a number of diseased states such as multiple sclerosis, sudden cardiac death, insulin resistance, atherosclerosis and hypertension (Blaak, 2003; Oram & Bornfeldt, 2004; Pilz et al., 2007; Sarafidis & Bakris, 2007; Hon et al., 2009a, 2009b). The fatty acid status may be assessed in plasma, platelets, red blood cells, white blood cells and brain ...