In vitro clonal propagation of Ashoka, Saraca asoca (Roxb.) De Wilde plant was achieved using shoot tip, nodal and internodal explants. The explants were cultured on MS medium supplemented with different concentrations (0.5-2.0mg/l) of Benzyl amino purine (BAP), Kinetin (Kn) and 2, 4-Dichlorophenoxy acetic acid (2,4-D). The synergetic effect of BAP (0.5mg/ l) induced a mean of 11.71±0.53 advent...

An efficient regeneration protocol was developed from shoot tip and nodal explants of Simarouba glauca DC, a promising biodiesel plant. Nodal explants appeared to have better regeneration capacity than shoot tip explants (40%) in the tested media. The highest regeneration frequency (90%) and shoot number (7.00 ± 1.00 shoots per explants) were obtained in nodal explants in Murashige and Skoog’s ...

Presence of pharmaceutically important alkaloids in various parts of the plant body made Rauvolfia serpentina an overexploited one and led to its inclusion in Red Data Book of India as an endangered species. Conventional propagation is beset with problems like poor seed viability, low seed germination rate and delayed rooting of cuttings. In vitro clonal propagation may therefore be beneficial ...

Conservation and propagation of species using biotechnologic tools-such as plant tissue culture-are relevant when natural propagation is hampered for various reasons. In vitro techniques allow mass multiplication and propagation under pathogen-free conditions but also override dependence on season for availability of plant material. Moreover, in vitro genetic manipulation of a species, invariab...

In vitro propagation of rose (Rosa indica) as well as rose breeding is a valuable economic enterprise and has received highest importance globally including Indian subcontinent. The development of new cultivars for commerce is faced with new challenges. In vitro micropropagation protocols of varieties of roses have been established but very few reports on the clonal propagation of local cultiva...

This paper describes a prime and easy-touse protocol for large-scale production of plant lets through shoot tip culture of Lantana camera, the plant having phytoremediation potential and the method is useful for the ex-situ conservation of other species important for phytoremediation. A simple micro-propagation method from nodal and shoot tip explants were reported here for Lantana camera, at c...

in vitro propagation of rose (rosa indica) as well as rose breeding is a valuable economic enterprise and has received highest importance globally including indian subcontinent. the development of new cultivars for commerce is faced with new challenges. in vitro micropropagation protocols of varieties of roses have been established but very few reports on the clonal propagation of local cultiva...

Dog rose (Rosa canina) has been using as a rootstock for ornamental roses also it is one of the medicinal plants. It can be propagated under in vitro conditions. After removing chilling requirement of buds, axillary wood buds of dog rose were cut in three nodal positions (lower, middle and terminal) on the stem and then explants after decontamination, cultured on Murashige and Skoog medium (MS ...

Nodal explants of Phyla nodiflora (L.) Greene, were cultured on Murashige and Skoog (MS) medium supplemented with different combination and concentration of cytokinins and auxins for multiple shoots regeneration. The maximum numbers of shoots were found in MS medium supplemented with2.5 mgl-16-benzylaminopurine (BA) and 0.5 mgl-1 kinetin (KN). Elongated shoots were transferred to rooting ...

Andrographis lineata Nees (Acanthaceae) is an endemic herb rich in flavonoids and diterpenoids which have pharmaceutical importance. Seedling explants source has not been studied for micropropagation and hence the present investigation has been taken up. An efficient protocol was developed for aseptic seed germination and in vitro shoot multiplication from different aseptic seedling explants. 3...