AIMS To screen a pair of primers suitable for denaturing gradient gel electrophoretic (DGGE) analysis of ruminal methanogenic Archaea and to detect the archaeal communities in the rumen of goat. METHODS AND RESULTS Nine primer pairs for 16S rDNA of methanogenic Archaea, including six for directed polymerase chain reaction (PCR) and three for nested PCR were first evaluated by PCR amplificatio...

Despite the widespread prevalence of canine parvovirus disease (CPV) in Iranian dog population,molecular diagnosis of CPV variants, and investigation of the trends of its genetic changes is a new effort. Inthis study 50 samples from dogs suspicious of infection with clinical signs of diarrhea and vomiting, and 25samples from dogs suspected of infection with general symptoms su...

today, it is nearly 25 years past from investigation of shiga toxigenic escherichia coli (stec) which is able to produce shiga toxins and cause different gastroenteritis. since incidence of gastroenteritis due to stec is increasing, it's necessary to develop rapid, specific and accurate procedures like pcr. in this study, we used pcr method to detect and identify stec in cultures of 55 escheric...

Background: Identifying regional types and evaluating the frequency of pneumococcal strains has become increasingly important especially in vaccination. The purpose of this study was the identification and frequency determination of our regional serotype and evaluation of the performance of recent type specific multiplex PCR for the diagnosis of streptococcus pneumonia serotypes. Methods: All ...

bread-making quality in hexaploeid wheat is a complex trait for breeding programs. high molecular weight glutenin subunits (hmw-gs) have an important effect on dough stability and elasticity. the glu-1 loci that are encoded these subunits are located on the long arms of group-1 homologues chromosomes of the a, b and d genomes. in this work we used pcr-based dna markers to distinguish wheat brea...

BISTRO-PRIMER TOOL TO DESIGN AND VALIDATE SPECIFIC PCR PRIMER PAIRS FOR PHYLOGENETIC ANALYSIS Praful Aggarwal Marquette University, 2011 Polymerase Chain Reaction is a widely used biological technique which helps in amplifying small quantities of DNA. These amplified DNA copies are then used in several other experiments like DNA sequencing, phylogenetic analysis, etc. PCR primers are short subs...

Background. DNA sequences and immunoreactivity associated with Simian virus 40 transforming factors, large T and small t antigens (SV40LTAg), suggestive of an aetiopathogenetic link have been identified in fresh frozen tissue of a high proportion of recent cases of pleural mesotheliomas from the United States, Italy and Germany. SV40 is not normally infective in man though it can transform huma...