background and aims: ndv (newcastle disease virus) is one of the viruses that cause disease in avian with severe economic losses in the poultry industry in many countries. fusion protein (f) which plays a major role in the virus pathogenicity contains several regions that have a role in the fusion process. mutation in the sequence of hr1 & hr2 regions of this protein prevents fusion of the viru...

experimental infection of muscovy ducks with v4 strain of newcastle disease virus was undertaken to determine the response of the ducks to the virus and the possibility of virus transmission to ducks and chickens in village like conditions. twelve ducks were randomly and equally divided into three groups of control, inoculated and in-contact. additionally, the chickens were placed into two grou...

background and aims: newcastle disease virus (ndv) is one of the major pathogen in poultry. vaccination is intended to control the disease as an effective solution nevertheless this virus is a growing threat to the poultry industry. f gene open reading frame (orf) from ndv is 1650 bp, encoding a protein of 553 amino acids that can induce protective immunity alone. the f glycoprotein on the surf...

newcastle disease virus (ndv) was isolated and identified for the first time from a flock of japanese quail suspected to nd in iran. birds of the flock aged variously, and some of them died from the disease. loss of appetite, weakness and decrease in egg production, diarrhea and nervous symptoms were the main clinical signs in the birds. hemorrhagic lesions of the intestinal tracts and proventr...

this experiment was conducted for evaluating the humoral immune responses induced by poly lactide-co-glycolide acid (plga) microspheres coupled inactivated newcastle disease virus (ndv) vaccine in comparison to an ‘in-house’ prepared inactivated and a live commercial vaccine. plg microparticles containing inactivated ndv were prepared by a double emulsion technique based on solvent evaporation ...

background: the specific antibody response against newcastle disease virus (ndv) in the blood serum of chickens hatched from eggs exposed to low dose gamma-radiation was studied. materials and methods: two groups of eggs of commercial meat chicken lines were irradiated with the dose of 0.30 gy 60co gamma-rays before incubation and on the 19th day of incubation, respectively. the same number of ...

Newcastle disease is one of the main concerns of poultry farmers. Detection of virulent strains of Newcastle disease virus (NDV) has a great impact on control measures against the disease. In this study RT-PCR was optimized in high sensitivity in order to differentiate the virulent from non-virulent NDV isolates directly in tissue homogenates. The vaccinal NDV strain and known field isolates we...

infectious bronchitis (ib) and newcastle disease (nd) are highly contagious and the most economically important diseases of the poultry affecting respiratory tract and causing economic losses in poultry industry throughout the world. in the present study, the simultaneous detection and differentiation of causative agents of these diseases were investigated using duplex-rt-pcr. rna was extracted...

increased susceptibility of birds to avian pathogens in intensive husbandry system has emphasized on necessity of improvement of innate and specific immune responses of birds by the fast establishment of a beneficial microflora and immune stimulator factors to guarantee healthy and low-price products. during this study, 192 one-day-old broiler chicks (ross-380) in four groups with three replica...