We investigated the relative importance of binding site occupancy and epitope specificity in antibody neutralization of human immunodeficiency virus (HIV) type 1 (HIV-1). The neutralization of a T-cell-line-adapted HIV-1 isolate (MN) was analyzed with a number of monovalent recombinant Fab fragments (Fabs) and monoclonal antibodies with a range of specificities covering all confirmed gp120-spec...

background: induced abortion is not only a serious threat for women’s health, but also a controversial topic for its ethical and moral problems. we aimed to evaluate the relationship between neutralization techniques and attempting to commit abortion in married women with unintended pregnancy. methods: after in-depth interviews with some women who had attempted abortion, neutralization themes w...

Generation and inhibition of activated factor IXa was studied in factor XIa-activated plasma containing 4 mmol/L free calcium ions and 20 mumol/L phospholipid (25 mol% phosphatidylserine/75 mol% phosphatidylcholine). Interference of other (activated) clotting factors with the factor IXa activity measurements could be avoided by using a highly specific and sensitive bioassay. Factor IXa generati...

background and aims: canine distemper (cd) is a deadly infectious disease of canidae family. cd is a multi-systemic viral disease and is specified by wide range of clinical symptoms. the manifestations are not always indicative of cd, therefore a laboratory confirmation is necessary for suspected cases. materials and methods: different clinical specimens of 19 cd suspected unvaccinated dogs wer...

Passive transfer of antibody may be useful for preexposure prophylaxis against biological agents used as weapons of terror, such as Bacillus anthracis. Studies were performed to evaluate the ability of anthrax antiprotective antigen (anti-PA) and antilethal factor (anti-LF) neutralizing monoclonal antibodies (mAbs) to protect against an anthrax lethal toxin (LeTx) challenge in a mouse model and...

Inhibition of prothrombinase by antithrombin 111 (ATIII) and heparin was investigated in a continuous-flow system. Phospholipid-coated capillaries, containing phospholipid-bound factor Xa and factor Va, were perfused with 1.0 pmol/L prothrombin and 0.5 nmol/L factor Va. At 25°C and a flow rate of 32 pL/min (shear rate 28 seconds-‘) the steady-state rates of prothrombin conversion depended linea...

Altogether 562 sheep and goat blood serum samples were collected from livestock located in Konya, Burdur, and their environs in Turkey. The samples were tested for bluetongue virus antibodies by ELISA and serum neutralization test (SNT). Fifty-four (17.1%) out of 315 sheep serum samples from Konya and 1 (1.5%) out of 66 sheep serum samples from Burdur were detected as seropositive. Seventy-thre...