there is evidence indicating that pomegranate juice contains many of the essential properties necessary to retain cell viability and cell proliferation. these properties indicate that pomegranate juice is a suitable storage medium for avulsed teeth. however, this idea has not yet been tested. in this study, the capacity of pomegranate juice (pj) as a storage medium for retaining avulsed teeth w...

AIM Despite its therapeutic value almost nothing is known about potential adverse health effects of Olea europea L. We therefore investigated the in vitro toxicity and genotoxicity of leaf extracts of this plant. MATERIAL AND METHODS Extracts from olive tree leaves were obtained from four different regions in Tunisia. We investigated the in vitro toxicity, genotoxicity and antigenotoxicity of...

The appearance of lysosomes and the distribution of lysosomal enzymes have been studied in a number of cell cultures exposed to viruses. Lysosomes were shown by fluorescence microscopy after vital staining with aminoacridines and light microscopy after vital staining with neutral red. The lysosomal enzymes studied histochemically in unfixed and fixed cells were acid phosphatase and 5-bromo-4-ch...

BACKGROUND The aim of this study was to compare human and animal skin irritation data with results of selected in vitro methods, including HET-CAM test, Neutral Red Release Assay, Neutral Red Uptake Assay and EpiOcular eye irritation test and with already existing data of eye irritation obtained from animal experiments. METHODS Chemicals employed in previous skin irritation validation studies...

the interaction between isatin-β-thiosemicarbazone (ibt) and calf thymus dna (ct-dna) was investigated in physiological buffer (ph 7.4) using neutral red (nr) dye as a spectral probe by uv–vis absorption and fluorescence spectroscopy, as well as viscosity measurements. the ibt is stabilized by intercalation in the dna ( k [ibt –dna] =1.03×105 m−1), and displaces the nr dye from the nr–dna comple...

Introduction: Infectious diseases are one of the main causes of death worldwide. This has driven scientists to invest in extraction and identification of antimicrobial agents from natural toxins and presentation of novel antibiotics and vaccines. The aim of the current study is to investigate the antibacterial and cytotoxicity effects of the protective antigen domain 4 (PAD4) from Bacillus anth...

UNLABELLED A cell viability assessment method based computer vision analysis of the uptake of neutral red dye was used to quantify cell membrane integrity in raw and processed parenchyma cells of onion tissues. The presence of stained vacuoles was used as an indicator of tonoplast membrane integrity and photomicrographs were acquired for microscopic image analysis and cell integrity quantificat...

Surfactants represent one of the most common constituents in topical pharmaceutical and cosmetic applications or cleansers. Since adverse skin and ocular reactions can be caused by them, it is important to evaluate damaging effects. Amino acid-based surfactants deserve particular attention because of their low toxicity and environmental friendly properties. New lysine derivative surfactants ass...

I investigations carried out in this laboratory have been concerned with the initial responses of the corneal stromal cells to injury. One reaction which occurs in the stromal cells within a few hours after injury to the cornea is the activation of the fibrocytes to take up the vital dye, neutral red.' This reaction does not occur in the normal corneal stromal cells of the rat, but cells activa...