نتایج جستجو برای: nested quantitative pcr
تعداد نتایج: 483813 فیلتر نتایج به سال:
AIM To quantify Toxoplasma gondii DNA using a specially constructed artificial template as competitor in a nested polymerase chain reaction (PCR). METHODS The diagnostic assay was a nested PCR employing four primers that amplify part of the single copy gene for the P30 major surface antigen in T gondii. An artificial competitor containing the four primer binding sites was made first by creati...
BACKGROUND Dengue fever results from infection with one or more of four different serotypes of dengue virus (DENV). Despite the widespread nature of this infection, available molecular diagnostics have significant limitations. The aim of this study was to develop a multiplex, real-time, reverse transcriptase-PCR (rRT-PCR) for the detection, quantitation, and serotyping of dengue viruses in a si...
An association between human papillomavirus (HPV) and cervical neoplasia has been widely reported and HPV DNA is commonly detected in cervical carcinoma tissues. However, estimates of the prevalence of HPV infection differs among various detection methods. Seventy cases of cervical carcinoma were screened for HPV 16 infection by Southern blot hybridisation (SBH) and nested polymerase chain reac...
BACKGROUND Current malaria diagnostic tests, including microscopy and antigen-detecting rapid tests, cannot reliably detect low-density infections. Molecular methods such as polymerase chain reaction (PCR) are highly sensitive but remain too complex for field deployment. A new commercial molecular assay based on loop-mediated isothermal amplification (LAMP) was assessed for field use. METHODS...
Real-time PCR was evaluated as a quantitative diagnostic method for Chlamydia pneumoniae infection using different respiratory samples. Real-time PCR had efficiency equal to or better than that of nested touchdown PCR. This study confirmed sputum as the best sampling material to detect an ongoing C. pneumoniae infection.
purpose: to ascertain whether the use of nested-pcr (nested polymerase chain reaction) technique leads to more rapid and accurate diagnosis of bacterial endophthalmitis. methods: the aqueous humor and vitreous samples of 24 eyes of 24 patients with bacterial endophthalmitis were evaluated by microscopy, diagnostic aerobic culture, and nested- pcr to detect the infectious agent. results: aerobic...
اسهال ویروسی گاو (bvd) یک عامل بیماری¬زا است که در گاوها ایجاد عفونت می¬کند و اهمیت اقتصادی در جهان دارد. این ویروس موجب خسارت¬های اقتصادی قابل توجه بر صنعت دام¬پروری می¬شود. در این پژوهش با کمک 3 روش متفاوت شناسایی ویروس با نانو ذرات طلا انجام گرفت و شرایط واکنش بهینه شد. این 3 روش شامل نانو ذرات اصلاح نشده، نانو ذرات اصلاح شده به کمک 2 پراب و تک پراب بود. در هر سه روش تغییر رنگ در نمونه های ک...
conclusions the study indicated that the rate of p. jirovecii infection is similar to those reported from asian, indian and african countries. the similarity of the genetic pattern between iranian and indian isolates is probably due to their close geographical proximity. patients and methods we used two nested pcr and pcr-rflp assays to amplify mt lsu rrna and dhps genes in 126 samples obtained...
cryptosporidiosis is a gastroenteric disease caused by the protozoan parasite cryptosporidium parvum. water–borne transmission of this organism has become more prevalent in recent years. current method for detection of c. parvum oocysts in water is immunofluoresence assay (ifa). the method is time consuming, laborious and particularly not–specific. it cannot determine the infectivity of detecte...
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