dear editor-in-chief we read with interest the study by khazaei et al. (1) in which the authors have nicely concluded that pcr is more sensitive test than ziehl-neelsen staining and histo-pathological examination for the diagnosis of tuberculosis (tb). they have rightly pointed to use pcr, selectively, in acidfast bacilli negative paucibacillary forms of tb. however, we intend to highlight few ...

Paratuberculosis caused by Mycobacterium avium subs. paratuberculosis a chronic, inflammatory and fatal disease of ruminants. The infection is characterized by chronic “subclinic” phase. Cattle in this phase is capable of infecting other animals in the herd. Although the presence of paratuberculosis has been known, the scientific studies on the disease and the prevalence appears to be scarced i...

background neuroblastoma is the most common extracranial malignant solid tumor in children under 5 years, and it is characterized by wide clinical and biological heterogeneity. n-myc oncogene amplification is considered to be one of the most important prognostic factors used to evaluate survival in these patients. objectives the aim of our study was to determine amplification of the n-myc oncog...

results 162 yeast species from 250 specimens were isolated in sabroud dextrose agar (sda) medium. 106 (65.4%) of them were germ tube formation positive, 86 (53%) chlamydoconidium formation positive and 101(62.4%) were pcr positive. yeast cells and mycelia were detected in the isolates on direct microscopic examination. c. albicans accounted for 66.04% of cases and 34% were non-c. albicans speci...

anaplasma bovis is a leukocytotropic agent of bovine anaplasmosis and there is no available information about molecular study on this agent in cattle of iran. in this study a total 150 cattle blood samples were collected from central part of iran. the presence of a. bovis examined using light microscopic detection and species-specific nested polymerase chain reaction (nested-pcr) based on 16s r...

Evidences from many studies suggested a polymerase chain reaction (PCR) as a valuable method for diagnosing infectious disease in the transplant recipients. We used this method for detection of Toxoplasma, gondii and human cytomegalovirus in blood specimens from patients after bone marrow or kidney transplantation. DNA of both infectious agents were detected using two separate sets of nested pr...