Cyst nematodes are globally important pathogens in agriculture. Their sedentary lifestyle and long-term association with the roots of host plants render cyst nematodes especially good targets for attack by parasitic fungi. In this context fungi were specifically isolated from nematode eggs of the cereal cyst nematode Heterodera filipjevi. Here, Ijuhya vitellina (Ascomycota, Hypocreales, Bionect...

Twenty-one isolates of 18 fungal species were tested on water agar for their pathogenicity to eggs of Heterodera glycines. An egg-parasitic index (EPI) for each of these fungi was recorded on a scale from 0 to 10, and hatch of nematode eggs was determined after exposure to the fungi on water agar for 3 weeks at 24 C. The EPI for Verticillium chlamydosporium was 7.6, and the fungus reduced hatch...

Intensive vegetable production areas were surveyed in the provinces of Almería (35 sites) and Barcelona (22 sites), Spain, to determine the incidence and identity of Meloidogyne spp. and of fungal parasites of nematode eggs. Two species of Meloidogyne were found in Almería-M. javanica (63% of the samples) and M. incognita (31%). Three species were found in Barcelona, including M. incognita (50%...

The purpose of this study was to assess the effects of increasing initial population density (Pi) of Meloidogyne incognita on nematode reproduction and growth of susceptible tomato (Lycopersicom esculentum cv. Round-41) at 25°C± 2 in growth chamber. 21-days-old tomato seedlings transplanted in13-cm dia. earthen pot was inoculated with Pi including 250, 500, 1000, 1500 eggs of M. incognita. Nema...

A nondestructive technique was developed to characterize and separate eggs of soybean cyst nematode, Heterodera glycines, by developmental stage using flow cytometry. Eggs from cysts cultured on susceptible soybean roots were suspended in 0.1% xanthan gum or 59% sucrose and loaded into either a Coulter EPICS 752 or EPICS 753 flow cytometer. Eggs were analyzed and sorted according to forward ang...

The root-knot nematode (Meloidogyne spp.) is one of the principal nematode pests of field and vegetable crops in the southern United States (6). Meloidogyne spp. are generally not uniformly distributed in naturally infested field sites, and sites frequently contain more than one species, thus complicating the evaluation of breeding lines for nematode resistance. To facilitate evaluation of nema...

The plant-endoparasitic nematode Anguina pacificae is presently one of the most destructive pests of Poa annua in Northern California, causing stem galls at the base of tillers in this annual bluegrass, resulting in yellow or brown patches and irregular surfaces on golf course putting greens. The objective of the study was to investigate the population dynamics of A. pacificae on two golf cours...

The incorporation of a rye (Secale cereale L.) cover crop into the soil prior to planting cotton (Gossypium hirsutum L.) has been shown to restrict damage caused by root-knot nematodes (Meloidogyne incognita (Kofoid and White) Chitwood). A greenhouse study was conducted to determine the duration of the effectiveness of rye decomposition in controlling root-knot nematode damage in relation to th...

Solid CO (dry ice) was added to pots containing soil that was infested either with eggs of the root-knot nematode, Meloidogyne incognita, or with tomato (Lycopersicon esculentum 'Rutgers') root fragments that were infected with various stages of the nematode. Two hours after dry ice was added, thermocouples in the soil recorded temperatures ranging from -15 degrees C to -59 degrees C. One day a...