For supporting the Brazilian bovine encephalitis surveillance program this study examined the differential diagnosis of Neospora caninum in central nervous system (CNS) by histological analysis (HE staining), immunohistochemistry (IHC), and nested-PCR using a set of primers from the Nc5 region of the genomic DNA and ITS1 region of the ribosomal DNA. A sample of 302 cattle presenting neurologica...

neospora caninum is a coccidian protozoan that causes abortion in dairy and beef cattle and neurological disorders in dogs and horses. to identify n. caninum oocysts in the dog feces the molecular approaches are known as sensitive methods that specifically detect the oocysts. in present study, a polymerase chain reaction (pcr) targeting n. caninum specific nc5 genomic fragment was performed to ...

BACKGROUND The aim of this study was to analyse the effects of an estradiol (E(2))-progesterone substitution protocol on the endometrial expression of estrogen-sensitive genes during the peri-implantation period. METHODS Peripheral blood and endometrial biopsies were obtained from 13 infertile women both in a natural cycle (NC), on days 5 and 7 after ovulation (NC5, NC7), and in an artificial...

The experiment was made on a Lohmann Brown classic hybrid from 32 to 42 weeks of age. There were used feeds containing various levels of Ca, P or phytase: NC1 and NC2 were commercial recipes, NC3 was supplemented with Phyzyme (500 TFU/kg), NC4 was supplemented with monosodium phosphate and NC5 with dicalcium phosphate according to nutrient requirements (NRC 1998). There was tested the possibili...

Neospora caninum is a coccidian protozoan that causes abortion in dairy and beef cattle and neurological disorders in dogs and horses. To identify N. caninum oocysts in the dog feces the molecular approaches are known as sensitive methods that specifically detect the oocysts. In present study, a polymerase chain reaction (PCR) targeting N. caninum specific Nc5 genomic fragment was performed to ...

Neospora caninum has been detected in several wild mammalian species, i.e., deer, coyotes, dingoes, and foxes. Farm-bred foxes were rarely reported to be affected by the parasite. In this study, we detected for the first time the infection of N.caninum in farm-bred young blue foxes (Alopex lagopus) in China. Neospora-like tissue cysts were observed in brains and kidneys of the foxes by histopat...

Neosporosis is an important cause of bovine abortion worldwide. Many aspects of transmission of Neospora caninum in nature are unknown. The white-tailed deer (Odocoileus virginianus) is considered one of the most important wildlife reservoirs of N. caninum in the USA. During the hunting seasons of 2008, 2009, and 2010, brains of 155 white-tailed deer fetuses were bioassayed in mice for protozoa...

In the first part of the present research, an R290/R600 mixture was tested as a drop-in substitute in a 20cubic-feet, single-evaporator, autodefrost, topmount, conventional domestic refrigerator/freezer. All the hardware remained the same, only the capillary tube was lengthened to achieve the optimum performance. The best result with an optimized R290/R600 blend was 6% savings compared to the b...

New molecular and thermal diffusion coefficients of binary mixtures of normal decane-normal alkanes and methylnaphthalene-normal alkanes are measured at atmospheric pressure and T = 25 degrees C. The normal alkanes used in this work include nC5-nC20. Thermal diffusion coefficients were measured in a thermogravitational column. Molecular diffusion coefficients were measured using an open-ended c...

Abstract Background Toxoplasma gondii and Neospora caninum are important protozoan parasites with worldwide distribution among warm-blooded animals. Moreover, T. is a zoonotic parasite that infects humans. Poultries intermediated hosts of N. . However, little known about the contamination poultry eggs these parasites. We aimed to investigate molecular frequency chicken, domestic duck, quail fro...