Abstract Native polyacrylamide gel electrophoresis (PAGE) is a powerful technique for protein complex separation that retains both their activity and structure. In photosynthetic research, native-PAGE particularly useful given complexes are generally large in size, ranging from 200 kD to 1 MD or more. Recently, it has been reported the addition of amphipol A8-35 solubilized samples improved sta...

Polyacrylamide Gel Electrophoresis (PAGE) is both an analytical and preparative tool widely used in protein analysis. Proteins are separated by PAGE in an electric field according to their size, shape and charge both in their native configuration (native PAGE) or in the presence of denaturing agents such as the ionic detergent sodium dodecyl sulfate (SDS-PAGE). A third type of electrophoretic t...

BACKGROUND Conventional immunoassays underestimate the urinary albumin concentration because intact albumin in urine exists in two forms, immunoreactive and immunochemically nonreactive. METHODS Urinary albumin concentration measured by HPLC (which measures total albumin, i.e., the sum of immunoreactive albumin + immunochemically nonreactive albumin) or RIA was compared with densitometric ana...

Due to its design and integration into webpages, native advertising is a constantly growing topic among web developers and marketing experts [4, 7]. Research has shown that native advertising is not simply a golden ticket for advertisers, and greatly relies on proper implementation to be effective. This study assess native advertising’s effectiveness in goal-driven web browsing compared to stan...

Tricine–SDS-PAGE is commonly used to separate proteins in the mass range 1–100 kDa. It is the preferred electrophoretic system for the resolution of proteins smaller than 30 kDa. The concentrations of acrylamide used in the gels are lower than in other electrophoretic systems. These lower concentrations facilitate electroblotting, which is particularly crucial for hydrophobic proteins. Tricine–...

Blue native PAGE (BN-PAGE) uses Coomassie dye rather than denaturing SDS to provide a negative charge to proteins for electrophoresis. As such, it is a useful assay for investigating native supramolecular membrane complexes without the need for cross-linking. As Bak and Bax oligomers form in the mitochondrial outer membrane, and they can be efficiently monitored by BN-PAGE. Furthermore, BN-PAGE...