in this research, samples were collected from three iranian native dogs and three wolves. whole-genome sequencing for each individual was performed using next-generation sequencing technology. all short reads were aligned to the reference genome using bwa tool. single-nucleotide polymorphisms (snps) and small insertions and deletions (indels) were detected using the genome analysis toolkit (gat...

It has been possible to duplicate in the hemophilic dog four of the major experiments which have suggested in humans an "anticephalin" hypothesis for the pathogenesis of hemophilia. The experiments in the dog have been considerably extended, as compared with the human experiments, by a variety of techniques. I. Asbestos was placed in contact with hemophilic dog plasma, and the clotting time bec...

Genetic variation among the individuals is considered as an important tool for conservation of livestock animals. This study was conducted to analyze the genetic variation, phylogenetic relationship and paternal lineages among Iranian dog populations using seventeen autosomal and Y chromosome-specific microsatellite markers. Total DNAs of the samples were extracted and applied for genotype anal...

To determine the genetic relationships among domestic dog breeds, we performed both a sequence comparison of mitochondrial DNA (mtDNA) and an amplified fragment length polymorphisms (AFLP) analysis. Three of four regions of mtDNA, cytochrome b, cytochrome oxidase subunit II, and 16S rRNA genes were highly homogeneous among dog breeds, whereas the other region, the control region, showed relativ...

Using polyethylene glycol-mediated fusion of ATP-ase-enriched (native) microsomes with red blood cells, we have delivered sarcoplasmic reticulum (SR) Ca-ATPase and kidney Na,K-ATPase into the mammalian erythrocyte membrane. Experiments involving delivery of the SR Ca-ATPase into human red cells were first carried out to assess the feasibility of the fusion protocol. Whereas there was little det...

Genetic variability in purebred dogs is known to be highly structured, with differences among breeds accounting for approximately 30% of the genetic variation. However, analysis of the genetic structure in non-cosmopolitan breeds and local populations is still limited. Nine Portuguese native dog breeds, and other peripheral dog populations (five) with regional affinities, were characterized usi...