نتایج جستجو برای: narl

تعداد نتایج: 133  

Journal: :Journal of bacteriology 1997
S B Williams V Stewart

Nitrate and nitrite control of anaerobic respiratory gene expression is mediated by dual two-component regulatory systems. The sensors NarX and NarQ each communicate nitrate and nitrite availability to the response regulators NarL and NarP. In the presence of nitrate, the NarX protein acts as a positive regulator ("kinase") of both NarL and NarP activity. In the presence of nitrite, the NarX pr...

Journal: :Journal of bacteriology 1991
M Choe W S Reznikoff

Genes that are expressed under anaerobic conditions were identified by operon fusion techniques with a hybrid bacteriophage of lambda and Mu, lambda placMu53, which creates transcriptional fusions to lacZY. Cells were screened for anaerobic expression on XG medium. Nine strains were selected, and the insertion point of the hybrid phage in each strain was mapped on the Escherichia coli chromosom...

Journal: :Biochemical Society transactions 2006
R N Whitehead J A Cole

The ability of Escherichia coli to use both nitrate and nitrite as terminal electron acceptors during anaerobic growth is mediated by the dual-acting two-component regulatory systems NarX-NarL and NarQ-NarP. In contrast, Neisseria gonorrhoeae responds only to nitrite: it expresses only NarQ-NarP. We have shown that although N. gonorrhoeae NarQ can phosphorylate E. coli NarL and NarP, the N. gon...

Journal: :Journal of bacteriology 2005
Ee-Been Goh Peggy J Bledsoe Li-Ling Chen Prasad Gyaneshwar Valley Stewart Michele M Igo

Hierarchical control ensures that facultative bacteria preferentially use the available respiratory electron acceptor with the most positive standard redox potential. Thus, nitrate is used before other electron acceptors such as fumarate for anaerobic respiration. Nitrate regulation is mediated by the NarX-NarL two-component system, which activates the transcription of operons encoding nitrate ...

Journal: :International microbiology : the official journal of the Spanish Society for Microbiology 2010
Laura Teixidó Pilar Cortés Anna Bigas Gerard Alvarez Jordi Barbé Susana Campoy

Anaerobic metabolism is controlled by several transcriptional regulators, including ArcA, Fnr, NarP, and NarL, with the Fnr and ArcA proteins sensitive to the cell's redox status. Specifically, the two-component ArcAB system is activated in response to the oxidation state of membrane-bound quinones, which are the central electron carriers of respiration. Fnr, by contrast, directly senses cellul...

Journal: :Nucleic acids research 1990
C A Cooney

Ehel cleaves the site GGC/GCC and is an isoschizomer of Narl (GG/CGCQ and Bbel (GGCGC/C) (1 -4) . Here I show that Ehel is methyl sensitive and will not cleave its CpG methylated site (GC^CGCC). Narl and Bbel are also CpG methyl sensitive (5—7). Ehel methyl sensitivity is shown by digesting mixtures of methylated X DNA and unmethylated plasmid DNA and viceversa (Figure 1). I also digest eukaryo...

Journal: :Journal of bacteriology 2000
H Wang R P Gunsalus

Escherichia coli possesses two distinct nitrite reductase enzymes encoded by the nrfA and nirB operons. The expression of each operon is induced during anaerobic cell growth conditions and is further modulated by the presence of either nitrite or nitrate in the cells' environment. To examine how each operon is expressed at low, intermediate, and high levels of either nitrate or nitrite, anaerob...

Journal: :Journal of bacteriology 2006
Douglas F Browning David J Lee Alan J Wolfe Jeffrey A Cole Stephen J W Busby

The Escherichia coli K-12 nrf operon promoter can be activated fully by the FNR protein (regulator of fumarate and nitrate reduction) binding to a site centered at position -41.5. FNR-dependent transcription is suppressed by integration host factor (IHF) binding at position -54, and this suppression is counteracted by binding of the NarL or NarP response regulator at position -74.5. The E. coli...

2015
Ralf Baumann Susanne Kaempfer Novel N. Chegou Wulf Oehlmann Ralf Spallek André G. Loxton Paul D. van Helden Gillian F. Black Mahavir Singh Gerhard Walzl

Elevated antibody responses to Mycobacterium tuberculosis antigens in individuals with latent infection (LTBI) have previously been linked to an increased risk for progression to active disease. Studies in the field focussed mainly on IgG antibodies. In the present study, IgA and/or IgG responses to the mycobacterial protein antigens AlaDH, NarL, 19 kDa, PstS3, and MPT83 were determined in a bl...

Journal: :Journal of bacteriology 1999
E Härtig U Schiek K U Vollack W G Zumft

Bacterial denitrification is expressed in response to the concurrent exogenous signals of low-oxygen tension and nitrate or one of its reduction products. The mechanism by which nitrate-dependent gene activation is effected was investigated in the denitrifying bacterium Pseudomonas stutzeri ATCC 14405. We have identified and isolated from this organism the chromosomal region encoding the two-co...

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