Tangential flow filtration membrane systems are employed for the isolation and concentration of extracellular vesicles. However, interfacial interactions between surface species influence flux performance. Here we propose a strategy aimed at introducing functional ligands over to improve separation process through combined size-exclusion affinity-based mechanisms, avoiding binding contaminants ...

Our goal is to obtain a comprehensive description of molecular processes occurring at cellular membranes in different biological functions. We aim at characterizing the complex organization and dynamics of the plasma membrane at single-molecule level, by developing analytic tools dedicated to Single-Particle Tracking (SPT) at high density: Multiple-Target Tracing (MTT). Single-molecule videomic...

Analytical gas-permeation models for predicting the separation process across  membranes (exit compositions and area requirement) constitutes an important and necessary step in understanding the overall performance of  membrane modules. But, the exact (numerical) solution methods suffer from the complexity of the solution. Therefore, solutions of nonlinear ordinary differential equations th...

Materials with specific nanometric layers are of great value in both theoretical and applied research. The nanometric layer could have a significant influence on the response to the mechanical loading. In this paper, the nanometric cutting on the layered systems of silicon has been studied by molecular dynamics. This kind of composite structure with amorphous layer and crystalline substrate is ...

This contribution highlights and compares some recent achievements in the use of k-space and real space imaging (scanning probe and wide-filed microscope techniques), when applied to a luminescent color center in diamond, known as nitrogen vacancy (NV) center. These techniques combined with the optically detected magnetic resonance of NV, provide a unique platform to achieve nanometric magnetic...

The proton pump Bacteriorhodopsin (BR) undergoes repeated photocycles including reversible conformational changes upon visible light illumination. Exploiting the sensitivity of infrared (IR) spectra to conformation, we have determined reaction kinetic parameters conductive intermediate M for wild-type protein and its slow mutant D96N during photocycle. Time-resolved IR micro-spectroscopy using ...

In this work, a simple and effective way to modify the support surface is developed and a nanostructure ceramic support to facilitate deposition of a defect-free overlying micro and meso (nano) porous membrane is obtained. To achieve high performance nanocomposite membranes, average pore size of outer surface of support was reduced by dip-coating in submicron and nano α-alumina slurries. In...

Nanometric cutting involves materials removal and deformation evolution in the surface at nanometer scale. At this length scale, atomistic simulation is a very useful tool to study the cutting process. In this study, large-scale molecular dynamics (MD) simulations with the model size up to 10 millions atoms have been performed to study three-dimensional nanometric cutting of copper. The EAM pot...