Microalga, Nannochloropsis oculata, is an important microorganism for feeding fish larvae. We develop a transgenic line of N. oculata that enbles to produce an antimicrobial peptide, bovine lactoferricin (LFB). An algae-codon-optimized cDNA of LFB was fused with a red fluorescent protein (DsRed) reporter and driven by a heat-inducible promoter, which is a heat shock protein 70A promoter combine...

BACKGROUND Phytoplankton cultures are widely used in aquaculture for a variety of applications, especially as feed for fish larvae. Phytoplankton cultures are usually grown in outdoor tanks using natural seawater and contain probiotic or potentially pathogenic bacteria. Some Roseobacter clade isolates suppress growth of the fish pathogen Vibrio anguillarum. However, most published information c...

17 The SS-type rotifer Brachionus rotundiformis is a common initial food for rearing fish 18 larvae with small mouth. However, there are commercially important fish species whose mouth 19 sizes are too small to feed on SS-type rotifers. In 2004, we isolated a small (body length= 82.7 20 ±10.9 μm; body width 40.5±6.4 μm), flexible, and iloricate rotifer, Proales similis from an 21 estuary in Oki...

Characterization of the feeding preferences of bivalve larvae would help improving the bivalve aquaculture and hatchery by providing appropriate microalgal diets. However, inaccurate and laborious identification and counting of microalgal species have challenged the selective feeding of bivalves. In the present study, we developed a highly specific and sensitive assay using quantitative polymer...

A matrix of photobioreactors integrated with metabolic sensors was used to examine the combined impact of light and temperature variations on the growth and physiology of the biofuel candidate microalgal species Nannochloropsis oculata. The experiments were performed with algal cultures maintained at a constant 20 °C versus a 15 °C to 25 °C diel temperature cycle, where light intensity also fol...

Four species of microalgae were evaluated at different temperatures for their ability to remove ammonia from intensive marine fish/shrimp culture systems. Growth rates were highest on the first of three days of culturing for marine microalgae Nannochloropsis oculata, Isochrysis aff. galbana, Chaetoceros muelleri, and Tetraselmis chui. This rate was used to compare both the growth and the total ...

Background: This study quantified the effect of four popular culture media in a biodiesel production study on the qualitative and quantitative lipid content, dry biomass, and lipid productivity of Nannochloropsis oculata. Methods: Culture of microalgae was done separately in Walne, F/2, Sato, and TMRL media. In the logarithmic and stationary growth phases, biomass production and lipid accumulat...

This short communication reports a pioneering research of using bacteria for simultaneous algal cell disruption and cell wall/membrane utilization. Microalgae are regarded as one of the most promising feedstock that can potentially address the twin challenges of energy security and environmental protection due to their fast growth rate, high lipid content and CO2 biofixation capabilities. Howev...

Global environmental changes, including ocean acidification, have been identified as a major threat to scleractinian corals. General predictions are that ocean acidification will be detrimental to reef growth and that 40 to more than 80 per cent of present-day reefs will decline during the next 50 years. Cold-water corals (CWCs) are thought to be strongly affected by changes in ocean acidificat...

A mathematical modeling of microalgae biomass is an essential step to optimize the biomass and lipid production rate and to reduce the cost of microalgal biodiesel production system. In the present study, kinetic studies were carried out to describe the growth and neutral lipid production of two marine microalgae Chlorella salina and Nannochloropsis oculata under the nitrogen-repleted and -depl...