Linear DNA molecules with covalently closed hairpin ends (telomeres) exist in a wide variety of organisms. Telomere resolution, a DNA breakage and reunion reaction in which replicated telomeres are processed into hairpin ends, is now known to be a common theme in poxviruses, Borrelia burgdorferi and Escherichia coli phage N15. Candidate proteins that may perform this reaction have recently been...

The incorporation of isotopic nitrogen from N15-aspartic acid, N15-glutamic acid, or N15(amide nitrogen)-glutamine into the nitrogens of the pyrimidine ring of hypoxanthine synthesized from glycine-l-Cl4 in pigeon liver extracts has been reported to be approximately equivalent to the incorporation of Cl4 from labeled glycine (1). It was proposed that biological similarity of the transaminating ...

introduction: neonatal jaundice is the most common cause of hospitalization.animal study demonstrated a decrease in serum bililrubin level after zinc treatment in hyperbilirubinemic rats. the objective of this study was the investigation of serum zinc level in the neonates with idiopatic .   materials and methods: a case-control study was undertaken on 263 neonates in a neonatal intensive care ...

During DNA replication by the λ-like bacteriophages, immature concatemeric DNA is produced by rolling circle replication. The concatemers are processed into mature chromosomes with cohesive ends, and packaged into prohead shells, during virion assembly. Cohesive ends are generated by the viral enzyme terminase, which introduces staggered nicks at cos, an approx. 200 bp-long sequence containing ...

Several mutations in the N terminus of the G-protein-coupled receptor rhodopsin disrupt NXS/T consensus sequences for N-linked glycosylation (located at N2 and N15) and cause sector retinitis pigmentosa in which the inferior retina preferentially degenerates. Here we examined the role of rhodopsin glycosylation in biosynthesis, trafficking, and retinal degeneration (RD) using transgenic Xenopus...

We explored the therapeutic effect of NCX 1000, a derivative of ursodeoxycholic acid (UDCA) with nitric oxide (NO) donating properties, alone or in combination with vitamin E, in an experimental model of NASH in the rat. Methods. A control group was fed a standard liquid diet (Control), and the NASH groups were fed a high-fat liquid diet for 12 weeks without (NASH) or with simultaneous daily ga...

Peptide fragments of self-proteins bound to major histocompatibility complex molecules within the thymus are important for positively selecting T cell receptor (TCR)-bearing CD4(+)CD8(+) double positive (DP) thymocytes for further maturation. The relationship between naturally processed thymic self-peptides and TCR-specific cognate peptides is unknown. Here we employ HPLC purification of peptid...

A field experiment was conducted during the winter (rabi) seasons of 2015–16 and 2016–17 at Punjab Agricultural University, Ludhiana, to study effect staggered sowing on growth indices, photosynthetic parameters productivity barley (Hordeum vulgare L.) in relation different cutting management. The laid out a split-plot design with 3 dates, viz. 15 October, 30 October November main plots 5 manag...