Nicotiana benthamiana was transformed with a taxadiene synthase (TS) gene for the de novo synthesis of taxadiene. The successfully transformed lines of N. benthamiana producing taxadiene was further cultivated for root culture to produce taxadiene in in vitro system. The roots of lines TSS-5, TSS-7, TSS-8 and TSS-10 accumulated 14.6 22.5 μg/g (dw) taxadiene. Methyl jasmonate treatment to the TS...

BACKGROUND Nonhost resistance is a generalized, durable, broad-spectrum resistance exhibited by plant species to a wide variety of microbial pathogens. Although nonhost resistance is an attractive breeding strategy, the molecular basis of this form of resistance remains unclear for many plant-microbe pathosystems, including interactions with the bacterial pathogen of rice, Xanthomonas oryzae pv...

Two different motifs in the coat protein (CP) of Plum pox virus (PPV) (R(3015)Q(3016), D(3059)) were mutated by replacing the respective amino acids with others possessing different chemical properties. The mutated CP genes were introduced into an infectious full-length clone of PPV (p35PPV-NAT) to investigate their influence on systemic infection of transgenic wild-type PPV CP-expressing and n...

Phospholipid signaling plays an important role in plant immune responses. Here, we isolated two phospholipase C4 (PLC4) orthologs the Nicotiana benthamiana genome, designated as N. PLC4-1 and PLC4-2 (NbPLC4-1 NbPLC4-2). We created NbPLC4-1- NbPLC4-2- silenced plants. Induction of hypersensitive response (HR), including HR cell death bacterial population reduction, was accelerated both NbPLC4-2-...

Responding to the need for recombinant acidic fibroblast growth factor in the pharmaceutical and cosmetic industries, we established a scalable expression system for recombinant human aFGF using transient and a DNA replicon vector expression in Nicotiana benthamiana. Recombinant human-acidic fibroblast growth factor was recovered following Agrobacterium infiltration of N. benthamiana. The optim...

Grapevine Algerian latent virus (GALV) is a member of the genus Tombusvirus in the Tombusviridae and infects not only woody perennial grapevine plant but also herbaceous Nicotiana benthamiana plant. In this study, we developed GALV-based gene expression and virus-induced gene silencing (VIGS) vectors in N. benthamiana. The GALV coat protein deletion vector, pGMG, was applied to express the repo...

The phytoene desaturase (PDS) encodes a crucial enzyme in the carotenoid biosynthesis pathway. Silencing or inhibiting PDS expression leads to appearance of mottled, chlorosis, albino leaves. In this study, CDS sequence EuPDS (Eucommia ulmoides Phytoene Desaturase) was first cloned and then silenced Nicotiana benthamiana. Result showed level leaves higher than that roots stems. N. benthamiana l...

Xanthan is the major exopolysaccharide secreted by Xanthomonas spp. Despite its diverse roles in bacterial pathogenesis of plants, little is known about the real implication of this molecule in Xanthomonas pathogenesis. In this study we show that in contrast to Xanthomonas campestris pv campestris strain 8004 (wild type), the xanthan minus mutant (strain 8397) and the mutant strain 8396, which ...

Ca(2+) signaling is an early and necessary event in plant immunity. The tomato (Solanum lycopersicum) kinase Pto triggers localized programmed cell death (PCD) upon recognition of Pseudomonas syringae effectors AvrPto or AvrPtoB. In a virus-induced gene silencing screen in Nicotiana benthamiana, we independently identified two components of a Ca(2+)-signaling system, Cbl10 (for calcineurin B-li...