1. The incorporation of methyl groups into histones from dimethylnitrosamine and from methionine was studied by injection of the labelled compounds, isolation of rat liver and kidney histones, and analysis of hydrolysates by column chromatography. 2. Labelled methionine gave rise to labelled in-N-methyl-lysine, di-in-N-methyl-lysine and an amino acid presumed to be omega-N-methyl-arginine. 3. A...

Iodoacetamide inactivates glycogen phosphorylase and causes it to dissociate into monomers of 92,500 molecular weight. Three -SH groups on each monomer are alkylated, but the first reacts as rapidly as do model compounds and its alkylation does not cause inactivation. The remaining two -SH groups are alkylated several hundred times slower, at rates comparable to the loss of activity. The three ...

The current article reports theoretical studies (DFT: B3LYP/6-31CG(d)) on the structure and alkylation reactions of the anions of some secondary N-nitrosocarbamates, a class of ambident nucleophiles whose chemistry has been little explored. Several anions (1–4), with an increasing size of the carbamate alkyl (aryl) group were investigated, in an attempt to establish the influence of the size of...