Thirty-six Mycobacterium paratuberculosis isolates of bovine, caprine, and ovine origins were evaluated by using gas-liquid chromatography (GLC), thin-layer chromatography (TLC), and BACTEC 7H12 Middlebrook TB medium (12A; Johnston Laboratories, Inc., Towson, Md.) in an effort to more rapidly differentiate this group of organisms from other mycobacteria. Bacterial suspensions (0.1 ml) were inoc...

Mycobactin S has been isolated from Mycobacterium smegmati8 and from Mycobacterium sp. Olitzky & Gershon, strain 2, and mycobactin H from M. thermore8i8tible; all three organisms were grown on synthetic media of low iron content. These two mycobactins are mixtures of compounds having the same nucleus but differing in their fatty side chains. The nucleus of mycobactin S has a chemical structure ...

1. A growth factor for Mycobacterium johnei has been isolated from Mycobacterium tuberculosis as an iron complex; it has been named ferric mycobactin T. 2. Ferric mycobactin T as isolated is a mixture of at least four closely related components differing only in their fatty acid side chains. 3. Mycobactin T resembles mycobactin P in structure but differs in the following respects: (a) the aroma...

To measure molecular changes underlying pathogen adaptation, we generated a searchable dataset of more than 12,000 mass spectrometry events, corresponding to lipids and small molecules that constitute a lipidome for Mycobacterium tuberculosis. Iron is essential for M. tuberculosis survival, and the organism imports this metal using mycobactin and carboxymycobactin siderophores. Detection of an ...

The pathogenicity of 40 strains of Mycobacterium avium, M. paratuberculosis, M. intracellulare and M. lepraemurium was investigated in chickens, rabbits, guinea-pigs, mice and calves. Mycobactin dependence and serological type were also determined. There was no evidence that mycobactin dependence was related to pathogenicity. Antigenic similarities were demonstrated between M. avium and M. para...

The mycobactin siderophore system is present in many Mycobacterium species, including M. tuberculosis and other clinically relevant mycobacteria. This siderophore system is believed to be utilized by both pathogenic and nonpathogenic mycobacteria for iron acquisition in both in vivo and ex vivo iron-limiting environments, respectively. Several M. tuberculosis genes located in a so-called mbt ge...

The efficiency of culture media was compared for the culture and subculture of very slowly growing acid-fast bacilli and spheroplast forms obtained from intestinal tissues of patients with Crohn's disease and ulcerative colitis and from controls without inflammatory bowel disease. Media were developed by modifying a nutrient broth medium based on veal infusion broth and yeast extract. We evalua...

In February 2000, Becton Dickinson Biosciences initiated a study to evaluate a potential new product, Herrold's Egg Yolk Agar with Mycobactin J and arnphotericin B, nalidixic acid, and vancomycin (BBL HEYA-MANV). The study involved laboratories which produced their own similar product, and compared the ability of the two media to support the growth of Mycobacterium avium subsp. paratuberculosis...

Colonies of Mycobacterium smegmatis LR222 on iron-limiting (0.1 micro M Fe) minimal medium agar fluoresce under UV light due to the accumulation in the cells of the deferri form of the siderophore mycobactin. Two mutants with little or no fluorescence, designated LUN8 and LUN9, were isolated by screening colonies of transposon (Tn611)-mutagenized M. smegmatis. Ferrimycobactin prepared from iron...

mycobacterium paratuberclosis  is one of the most important of   micobacterium tuberculosis complex family. this pathogen can infect all ruminant animals like cattle , sheep and goat .  this research was done  with  cooperation of esfahan agricultral research center & veterinary organization  isfahan  & karaj razi research institute. the samples put in safe packing and cold position (beside ice...