Experimental autoimmune vitiligo was induced by the intradermal injection of the purified mushroom tyrosinase emulsified in Complete Freunds Adjuvant (CFA) in female C57BL/6 mice. The onset of vitiligo was characterized by hair hypopigmentation and total melanocyte depletion in the basal layer of the epidermis. Oral administration of semipurified mushroom tyrosinase prevented experimental autoi...

This study reports depigmenting potency of 1,3-selenazol-4-one derivatives, which would be based upon the finding of direct inhibition to mushroom tyrosinase. 1,3-Selenazol-4-one derivatives exhibited inhibitory effect on dopa oxidase activity of mushroom tyrosinase. In this study, inhibitory effects of six kinds of 1,3-selenazol-4-one derivatives (A, B, C, D, E and F) on mushroom tyrosinase we...

Rosmarinic acid isolated from the Perilla frutescens (L.) was found to possess mushroom tyrosinase (EC 1.14.18.1) inhibitory activities with an IC50 of 4.0 μM. However, rosmarinic acid was oxidized as a substrate catalyzed by tyrosinase. The oxidation kinetics of mushroom tyrosinase by rosmarinic acid measured by spectrophotometic and polarographic methods showed Km=166.8 μM, 225.1 μM and Vmax=...

the present study was designed to investigate the anti-melanogenic and cytotoxic activities of methanol extract of phlomis kurdica. the antioxidant and anti-tyrosinase activity of meoh extract from p. kurdica (mpk) were examined by dpph radical scavenging and mushroom tyrosinase activity assays (in vitro), respectively. furthermore, the effect of mpk on the melanin content, cellular tyrosinase ...

tyrosinase has been recognized as a major autoantign in human vitiligo. here we have shown that experimental autoimmune vitiligo can be induced by intradermal injection of mushroom tyrosinase emulsified in complete freund’s adjuvant in c57bl/6 mice. the onse of vitiligo was characterized by hair hypopigmentation and total melanocyte depletion in the basal layer of the epidermis. our results con...

Tyrosinase, a widely spread enzyme in micro-organisms, animals, and plants, participates in two rate-limiting steps in melanin formation pathway which is responsible for skin protection against UV lights' harm whose functional deficiency result in serious dermatological diseases. This enzyme seems to be responsible for neuromelanin formation in human brain as well. In plants, the enzyme leads t...

ETHNOPHARMACOLOGICAL RELEVANCE Sargassum polycystum, a type of brown seaweed, has been used for the treatment of skin-related disorders in traditional medicine. AIM OF THE STUDY The aim of the present study is to investigate the antimelanogenesis effect of Sargassum polycystum extracts by cell-free mushroom tyrosinase assay followed by cell viability assay, cellular tyrosinase assay and melan...

Tyrosinase is a key enzyme in melanogenesis. Generally, mushroom tyrosinase from A. bisporus had been used as a model in skin-whitening agent tests employed in the cosmetic industry. The recently obtained crystal structure of bacterial tyrosinase from B. megaterium has high similarity (33.5%) to the human enzyme and thus it was used as a template for constructing of the human model. Binding of ...

Cutaneous hyperpigmentation is a common disorder due to excess melanin production by the enzyme tyrosinase. The gold standard for treatment is hydroquinone (HQ), which reduces pigmentation through its toxicity to melanocytes rather than via tyrosinase inhibition. We screened an internal library for oligopeptides that inhibited both mushroom and human tyrosinase but showed no cytotoxicity to hum...

The  inhibition effects seed and peel of melon on diphenolase activity of mashroom tyrosinase were investigated. The IC50 values and Ki values of seed and peel of melon were evaluated. increased the Km value and decreased the Vm value so it show mixed type inhibition on mushroom tyrosinase when L-DOPA was used as a substrate.