background : avian influenza virus (aiv) infection is a major cause of bird and human morbidity and mortality. we aimed to evaluate a specific and sensitive multiplex rt-pcr that can simultaneously detect influenza type a viruses and differenti­ate the two most important subtypes of avian influenza viruses h7 and h9 subtypes. methods : a multiplex reverse transcriptase-polymerase chain reaction...

Background and Aims: Infectious bronchitis virus (IBV) causes an acute, highly contagious respiratory and kidney disease of chickens which results in significant economic losses in commercial broilers, layers and breeders. Rapid identification of IBV serotypes involved in respiratory complex is a problem in the differential diagnosis. Materials and Methods: In this study, a single and mul...

avian influenza virus (aiv) infection is a major cause of bird or human mortality and morbidity, therefore the rapid identification of the virus is of important clinical and epidemiological implication. methods: a multiplex reverse transcriptase pcr (rt-pcr) was optimized for the detection of influenza a virus and the h5 and h9 subtypes. the influenza type a specific primers were directed to th...

This study firstly developed a multiplex real-time PCR (RT-PCR) technique combined with a pre-enrichment step to simultaneously detect Staphylococcus aureus (S. aureus), Listeria monocytogenes (L. monocytogenes) and Salmonella spp. in raw milk and the dairy farm environment (feces, soil, feed, water) in one reaction. Brain heart infusion (BHI) broth was selected for the enrichment step to incre...

BACKGROUND Characterization of fusion gene transcripts in leukemia that result from chromosome translocations provides valuable information regarding appropriate treatment and prognosis. However, screening for multiple fusion gene transcripts is difficult with conventional PCR and state-of-the-art real-time PCR and high-density microarrays. METHODS We developed a multiplex reverse transcripti...

BACKGROUND Avian influenza virus (AIV) infection is a major cause of bird or human mortality and morbidity, therefore the rapid identification of the virus is of important clinical and epidemiological implication. METHODS A multiplex Reverse Transcriptase PCR (RT-PCR) was optimized for the detection of influenza A virus and the H5 and H9 subtypes. The influenza type A specific primers were di...

A novel multiplex reverse transcription polymerase chain reaction (multiplex RT-PCR) that can detect porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV), and porcine group A rotavirus (GAR) was developed. The 3 viruses (PEDV, TGEV, and porcine GAR) are major agents in viral enteric diseases of piglets. As the clinical signs of these diseases are similar, including...

Human enterovirus 71 (HEV71) and coxsackievirus A16 (CVA16) are two major aetiological agents of hand, foot and mouth disease (HFMD) in children. Recently there have been several large outbreaks of HFMD in Vietnam and the Asia-Pacific region. In this study, a multiplex RT-PCR assay was developed in order to detect simultaneously HEV71, CVA16 and other human enteroviruses. Enterovirus detection ...

Dear Editor The new 2008 WHO classification of hemato-oncological diseases includes a new category for recurrent chromosomal abnormalities [1]. Recurrent gene rearrangements such as RUNX1/ RUNX1T1 (formerly AML1/ETO), CBFB/MYH11, or PML/RARA, provide additional information for the diagnosis of AML, regardless of the blast count. Chromosomal abnormalities and gene mutations are the most influent...