The present study assessed the diagnostic usefulness of a multiplex PCR (mPCR) for Streptococcus pneumoniae, Haemophilus influenzae, Mycoplasma pneumoniae and Chlamydophila pneumoniae applied to bronchoalveolar lavage (BAL). Fibreoptic bronchoscopy was performed on 156 hospitalised adult patients with lower respiratory tract infection (LRTI) and 36 controls. BAL fluid was analysed with bacteria...

The kinesin is the main motor protein in the trafficking system of the mitochondria and other organelles. Genetic variants affecting the function of kinesin were earlier found to be associated with cognitive disturbances and neurodegeneration. A multiplex polymerase chain reaction (mPCR) method, allowing the simultaneous detection of the KNS2 A185C (rs_1054080) and C406T (rs_3742465) mutations ...

salmonellosis as an important zoonotic disease that causes food born poisoning in human through animalproducts and is considered as a worldwide public health hazard. widespread studies have been conducted ondifferent aspects of incidence, treatment and control of salmonelosis all over the world. the aim of this studywas to evaluate the occurrence of widespread salmonella serovars, s. typhimuriu...

Purpose Identification of bacteria and susceptibility are fundamental in periprosthetic joint infection (PJI). Especially in the case of systemic inflammatory response syndrome (SIRS) rapid detection of pathogens is essential for proper therapy. Bacterial cultures are time consuming. The polymerase chain reaction (PCR) is a non-culture molecular method and is able to rapidly identify pathogens ...

A specific and sensitive multiplex PCR (mPCR) method was developed as a useful tool for the simultaneous detection of two important flatfish pathogens in marine aquaculture, Tenacibaculum maritimum and Edwardsiella tarda. In fish tissues, the average detection limit for these mPCR-amplified organisms was 2 × 10 ⁵ ± 0.2 CFU/g and 4 × 10 ⁵ ± 0.3 CFU/g, respectively. These values are similar or ev...

Avian leukosis virus (ALV) subgroups A, B, and J are very common in poultry flocks and have caused serious economic losses in recent years. A multiplex PCR (mPCR) method for the detection of these three subgroups was developed and optimized in this study. We first designed a common forward primer, PF, and three downstream primers, AR, BR, and JR, which can amplify 715 bp for subgroup A, 515 bp ...

BACKGROUND Bacterial meningitis is more common in the neonatal period than any other time in life; however, it is still a challenge for the evidence based diagnosis. Strategy for identification of neonatal bacterial meningitis pathogens is presented by evaluating three different available methods to establish evidence-based diagnosis for neonatal bacterial meningitis. METHODS The cerebrospina...

Indonesia become a maritime country with large of ocean area and beach line marine species diversity reaches 37% the world’s fish. This makes have aquaculture potential that supported by an appropriate climate. However, challenge viral diseases caused Infectious Spleen Kidney Necrosis Virus (ISKNV) Viral Nervous (VNN) would hamper causing huge economic losses. Therefore, it is necessary to carr...

Fishery products are types of commodity with various levels development including the addition food additives. They very susceptible to patogenic microbial contamination hence they can cause poisoning and disease outbreaks in consumers. In this study, tuna fish spiked Salmonella spp., Escherichia coli, Listeria monocytogenes bacteria is examined for contamination. The examination these carried ...