Two colorimetric methods that use Alamar Blue or 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) for assaying the in vitro activities of antifungal agents have been described. We report that both tests performed similarly when the antifungal activity of amphotericin B against Candida albicans was determined. However, only the MTT test generated interpretable data when Asp...

Objective: To determine the phytochemical constituents, antioxidant and anticancer activities of Macranga peltata leaf extracts on A549 P 51 human lung cancer cell lines.
 Methods: Leaf sample was subjected to soxhlet extraction method screening done using different biochemical tests. The activity tested 2,2–diphenyl-1-picrylhydrazyl, ferric ion reducing power assay 2,2–azino bis 3–ethyl b...

Colorectal cancer is one of the most diagnosed cancers in world. KRAS mutations colon are being responsible for progressiveness and resistance standard therapeutic available. Marine sponge sources chemotherapy. Stylissa carteri a marine that lives Indonesia its anti-cancer effects starting to be explored nowadays. The purpose this study was determine cytotoxicity anti-proliferative effect ethan...

Abstract This work evaluates the in vitro antioxidant and antidiabetic activities of two metformin hydrochloride-based Schiff bases. Moreover, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay was used to examine cytotoxic effects HL1 HL2 on A549 lung cancer cell line. The bases that have been previously synthesized by using effective, green techniques, namely stirring m...

Objective: The current study emphasizes the effects of stem and leaf methanolic extract mangrove plant Avicennia alba on human tumor cell lines HeLa MCF-7.
 Method: collected sample was subjected to extraction with methanol screened for anticancer activity according 3-(4,5-dimethythiazol- 2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay method. ability inhibit proliferation MCF-7 procure...

Complex formation and liquid-liquid extraction were studied in systems containing iron(III), 4-nitrocatechol (4NC),tetrazolium salt (TZS), water and organic solvent. Three different TZS were used: 3-(4,5-dimethyl-2-thiazol)-2,5-diphenyl-2H-tetrazolium bromide (MTT), 3-(2-naphtyl)-2,5diphenyl-2H-tetrazolium chloride (Tetrazolium violet, TV) and 2-(4-iodophenyl)-3-(4-nitrophenyl)5-phenyl-2H-tetra...

Cytotoxicity of the futuristic nanogenomedicine (e.g., short interfering RNA and antisense) may hamper its clinical development. Of these, the gene-based medicine and/or its carrier may elicit cellular toxicity. For assessment of such cytotoxicity, a common methodology is largely dependent upon utilization of the 3-(4, 5-Dimethyl-2-thiazolyl)-2, 5-diphenyl-2H-tetrazolium bromide (MTT) assay whi...

Poly(ADP-ribose)polymerase (PARP) is a significant therapeutic target for the treatment of numerous human diseases. Olaparib has been approved as PARP inhibitor. In this paper, series new compounds were designed and synthesized with lead compound. order to evaluate inhibitory activities against PARP1 compounds, in vitro inhibition assay intracellular PARylation conducted. The results showed tha...

Solanum nigrum is used in various traditional medical systems for antiproliferative, antiinflammatory, antiseizure and hepatoprotective activities. We have evaluated organic solvent and aqueous extracts obtained from berries of Solanum nigrum for antiproliferative activity on leukemic cell lines, Jurkat and HL-60 (Human promyelocytic leukemia cells). The cell viability after the treatment with ...