Objective(s): Here, we examined the function of our produced monoclonal antibody (mAb10C3) to recognize one of the most important members of the HEAT shock factor family, Hsf5, in embryonic development and in spermatogenic cells of adult mouse testis.Materials and Methods: The targeting effects of mAb10C3 were investigated by immunohisto...

Background: Disruption in spermatogenesis and infertility occurred after local testicular heating. In this study in addition to evaluation the long term effects of experimental bilateral and unilateral cryptorchidism on sperm parameters and structure of mouse testis, the genes that involved in programmed cell death after cryptorchidism and treatment methods of decreasing the germ cells in semin...

Cbfa1 is an essential transcription factor for bone formation, but its transcripts have also been detected in thymus and testis. To elucidate the expression of Cbfa1 in testis, we isolated Cbfa1 cDNA from mouse testis. First we examined the length of the transcripts expressed in mouse testis by Northern hybridization. Using a cDNA probe that can detect both Type-I and Type-II Cbfa1 (Type-I: ori...

The expression of gonadotrophin-releasing hormone receptor (GnRH-R) in germinal cells of mouse testis, whole testes, pituitary glands, and mouse ovaries was determined by means of Northern hybridization using a mouse GnRH-R [(32)P]-labelled cDNA probe. Also, the expression of GnRH-R in rat germinal cells, testis and pituitary gland was determined by Northern blot analysis using the same mouse-s...

In all vertebrates sex determination is the step at which development of a testis or ovary is initiated in the bipotential gonad. Although Mus musculus and the red-eared slider turtle, Trachemys scripta, use different mechanisms to initiate organogenesis of the testis (the Y-linked gene, Sry, in the mouse vs. the incubation temperature of the egg in the turtle), the structure of the adult testi...

Background: Doxorubicin (DOX), a widely used antineoplastic compound, is associated with testicular damage and infertility. In this study, we intended to determine the possible preventive effects of simvastatin (SIM), a lipid lowering agent with antioxidant and anti-inflammatory activities, on testicular morphometric-stereological changes due to DOX treatment in mice. Materials and Methods: Mal...

Alternative pre-mRNA splicing (AS) is a key molecular event that allows for protein diversity and plays important roles in development and disease. AS regulations during spermatogenesis and AS aetiology in testicular tumorigenesis have not yet to be characterized. By genome-wide analysis, here we describe alternative splicing features that distinguish distinctive patterns of AS among human test...

Various histones, including testis-specific histones, exist during spermatogenesis and some of them have been reported to play a key role in chromatin remodeling. Mass spectrometry (MS)-based characterization has become the important step to understand histone structures. Although individual histones or partial histone variant groups have been characterized, the comprehensive analysis of histon...

Abstract Testicular development and function rely on interactions between somatic cells the germline, but similar to other organs, regenerative capacity declines in aging disease. Whether adult testis maintains a reserve progenitor population remains uncertain. Here, we characterize recently identified mouse interstitial expressing transcription factor Tcf21. We found that TCF21 lin are bipoten...