The aim of this study was to evaluate the effect of four different storage media on the elution of monomers from two composite materials. Samples (n = 10, diameter: 4.5 mm, thickness: 2 mm) of two different composite materials (Ceram X™ & Filtek™ Supreme XT) were stored after polymerization in four different media (NaCl, saliva, ethanol 75% & acetone) for 24 h, 7 days, and 28 days. From the sto...

Proteoglycan biosynthesis by human osteochondrophytic spurs (osteophytes) obtained from osteoarthritic femoral heads at the time of surgical joint replacement was studied under defined culture conditions in vitro. Osteophytes were primarily present in two anatomic locations, marginal and epi-articular. Minced tissue slices were incubated in the presence of [(35)S]sulphate or [(14)C]glucosamine....

AIM This study was designed to determine the type and amount of the monomers leached from the different particle sizes of the composite materials. MATERIALS AND METHODS Three different disk sizes (2, 4, 6 mm) prepared for each material group (Filtek Flow, Filtek A110, Filtek P60 and Filtek Supreme) were polymerised by LED and halogen light; the specimens were then placed in artificial saliva....

OBJECTIVE The aim of this study was to assess the effect of bleaching on elution of monomers from nanofilled and microhybrid composites. MATERIALS AND METHODS 80 samples (5mm diameter and 3mm thickness) of each composite were prepared. After curing, half of them were randomly polished. Each group was divided into 8 subgroups and immersed in water or 10%, 20% and 30% H2O2 for 3 or 8 hours. Elu...

The present study was aimed to evaluate the effects of food simulating liquids and thermocycling on the elution of monomers from a nanofilled resin composite in different immersion times. Five Specimen discs were made from a nano-hybrid composite (Supreme 3M) for each group (Total = 180) and immersed in distilled water (control), citric acid, lactic acid, and 75% aqueous ethanol solution. The d...

OBJECTIVES To test the null hypotheses that photoactive resin composites containing a Type I photoinitiator would exhibit reduced DC or increased monomer elution at substantially short curing times compared with materials based on a Type 2 ketone/amine system. METHODS Two experimental resin composites were prepared, using either Lucirin-TPO or camphorquinone/DMAEMA. Specimens were light-cured...

Myoblasts and fibroblasts of embryonic skeletal muscle reproducibly form chondrocytes when cultured on demineralized bone in vitro. The transformation occurs in 3 morphologically defined phases, with disappearance of the myoblast phenotype preceding the appearance of fibroblast-like cells and finally chondrocytes. Proteoglycan synthesis in these cultures was investigated by labeling at prechond...

1. Delipidation of the Ca2+-ATPase of sarcoplasmic reticulum membranes by gel chromatography employing ionic detergents (cholate, deoxycholate and mixtures of both) in the presence of glycerol has been studied with respect to residual phospholipids and ATPase activities. 2. The extent of delipidation depends on the detergent chosen and on the ionic strength of the elution buffer. Increasing ion...