نتایج جستجو برای: monolayer cell culture
تعداد نتایج: 1881478 فیلتر نتایج به سال:
we aimed to develop a cost-effective and robust method to predict drug resistance in individualpatients. representative tissue fragments were obtained from tumors removed from femalepatients, aged 24-74 years old. the tumor tissue was taken by a histopathology’s or a surgeonunder sterile conditions. cells obtained by enzymatic dissociation from tumor after surgery, werecultured as a monolayer f...
Background: While articular chondrocytes are among those appropriate candidates for cartilage regeneration, the cell dedifferentiation during monolayer culture has limited their application. Several investigations have indicated the usefulness of alginate, but the topic of proliferation and differentiation of chondrocytes in alginate culture has still remained controversial. Methods: Rat articu...
background: the problem of bone defects during treatments of bone lesions, especially cranial trauma, and also during craniotomy operations indicates isolation of osteoblasts and culturing them for filling these bone effects. methods: bone tissue specimens were obtained from 6 patients’ carvaria undergoing craniotomy surgery operations in al-zahra teaching hospital and transferred to lab. these...
Objective Genetic and morphologic similarities between mouse embryonic stem cell (ESCs) and Primordial Germ Cell (PGCs) make it difficult to distinguish the two cell types in in vitro differentiation. Using the expression of specific markers of germ cells that are not expressed or expressed at low levels in ESCs, can help recognizing in vitro differentiated cells MaterialsAndMethods In this stu...
Background: The buffalo (Bubalus bubalis) is the animal with special production characteristics in producing milk and meat has a critical role in rural industry in Iran. The most problem of this specie is its low reproductive output. The aim of the present study was to evaluate the effect of bovine cumulus co-culture on maturation rate of Buffalo oocyte.Materials and Methods: Ovaries were colle...
many cell lines, when cultured under proper conditions, can form three dimensionalstructures called multicellular spheroids. these spheroids resemble in vivo tumor models in severalaspects. therefore, studying growth characteristics and behavior of spheroids is beneficial inunderstanding the behavior of tumors under various experimental conditions. in this work, we havestudied the growth proper...
Purpose: The objective for this study is to investigate the morphology and ultrastructure of mouse endometrial epithelial cell monolayer cultured on matrigel in dual-chambered system as an in vitro mouse endometrial epithelial cell culture model that mimics structural and functional properties of the endometrial epithelium in vivo. Materials and Methods: Mouse endometrial epithelial cells were...
We aimed to develop a cost-effective and robust method to predict drug resistance in individualpatients. Representative tissue fragments were obtained from tumors removed from femalepatients, aged 24-74 years old. The tumor tissue was taken by a histopathology’s or a surgeonunder sterile conditions. Cells obtained by enzymatic dissociation from tumor after surgery, werecultured as a monolayer f...
We aimed to develop a cost-effective and robust method to predict drug resistance in individualpatients. Representative tissue fragments were obtained from tumors removed from femalepatients, aged 24-74 years old. The tumor tissue was taken by a histopathology’s or a surgeonunder sterile conditions. Cells obtained by enzymatic dissociation from tumor after surgery, werecultured as a monolayer f...
Background and purpose: Despite the rapid development of assisted reproduction techniques (ÂRT) in recent years, implantation rates after replacement of embryos into the uterine cavity remains low. Ân important approach to improve embryo quaility and implantation rates has been the use of coculture systems. Hence this study was undertaken to evaluate the influence of cumulus cells monolayer i...
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