there are many studies related to the production of a elisa kit for diagnosing virus infections. however, production of most kits depends on purification of whole virus particles, which involves the use of costly equipment and reagents. the purpose of this study was to check out if the anti-cp42 antibodies could be used as a diagnostic assay for detection of grapevine fanleaf virus (gflv). in t...

RhD antigen is the most immunogenic and clinically significant antigen of red blood cells after ABO system. It has historically been associated with hemolytic disease of the newborn (HDN) which is now routinely prevented by the administration of polyclonal anti-D immunoglobulin. This management of HDN has proven to be one of the most successful cases of prophylactic treatment based on antibody ...

Previous reports have shown the A/J anti-para-azophenylarsonate (anti-Ar) antibodies that share a major cross-reactive idiotype (CRI) comprise a family of closely related but nonidentical molecules. Serological studies with CRI+ monoclonal anti-Ar antibodies have suggested the presence of a conserved idiotypic determinant within the family. The present study utilized monoclonal ant-idiotypic de...

Adhesion that is mediated by integrins is controlled dynamically to allow cell positioning and migration and to prevent abnormal trafficking and anchorage. Integrin signalling in response to ligand binding is achieved by a combination of receptor clustering and conformational changes. Both of these processes can be elicited from the inside of the cell (resulting in the acquisition of a highaffi...

CD19 is expressed on B- lineage cells and follicular dendritic cells and plays a key role in B cell malignancies and autoimmune diseases. Thus, it has been considered as potential target for several monoclonal antibodies (mAbs). For decades, chemotherapy has been known as one of the major antitumor therapies eradicating high proliferative tumor cells. But, anti- CD19 mAbs developed for treating...

Mucins are of major interest in cell biology, not only are they highly over-expressed in many adenocarcinomas (up to 40-fold increase), but also have important physiological function, and probably more to be determined (1-3). There is much information available on mucins - doubtless because of their unusual structure being heavily glycosylated, but also containing a repeat region rich in the am...

Recently, we have shown that anti-BMP2 monoclonal antibodies (mAbs) can trap endogenous osteogenic BMP ligands, which can in turn mediate osteodifferentiation of progenitor cells. The effectiveness of this strategy requires the availability of the anti-BMP-2 monoclonal antibodies antigen-binding sites for anti-BMP-2 monoclonal antibodies to bind to the scaffold through a domain that will leave ...

introduction: the present study was undertaken to produce alkaline phosphatase anti- alkaline phosphatase (apaap) complex and to use it in one of the most practical methods of determining the site of antigen in tissue or in cell, i.e. apaap technique, and its comparison with similar foreign products. materials and methods: in this basic and applied study the secreted antibodies of the two hybri...