A simple, sensitive Spectrophotometric determination of monoamine oxidase activity from tissue homogenates is described. This method is based on the high extinction coefficient of 4-hydroxy-3-methoxy-benzaldehyde (vanillin) at 345 nm at pH ~ 11.7. Vanillin is formed by inonoamine oxidase when 4-hydroxy-3-methoxy-benzylamine is used as substrate. The primary oxidation product, 4-hydroxy-3-methox...

Dose-response curves of pupillary dilation after topical administration of norepinephrine or methoxamine have been determined in rabbits after chronic inhibition of ocular monoamine oxidase by treatment with pargyline or pheniprazine. Eyes treated with either monoamine oxidase inhibitor showed an enhanced responsiveness to the mydriatic effect of norepinephrine given either topically or intrave...

It is well established that tobacco smokers have reduced levels of monoamine oxidase activities both in the brain and peripheral organs. Furthermore, extensive evidence suggests that smokers are less prone to develop Parkinson's disease. These facts, plus the observation that inhibition of monoamine oxidase B protects against the parkinsonian inducing effects of the nigrostriatal neurotoxin 1-m...

Historically, much of the focus on monoamine oxidases and their substrates has been in the area of depression and the monoamine neurotransmitters serotonin (5-hydroxytryptamine), noradrenaline, and to a lesser extent, dopamine. With both forms of monoamine oxidase (A and B), the production of hydrogen peroxide as a byproduct of the reaction between the monoamine oxidases and their monoamine sub...

drug clorgyline. Type B enzyme (or enzymes) deaminates only dopamine and is inhibited by deprenyl. The clinical papers include presentations and discussion by pioneers in the use of monoamine oxidase inhibitors in clinical states. Pare's observation that some bipolar depressions respond only to monoamine oxidase inhibitors is in accord with some clinical observations made at Yale by Himmelhoch ...

The aim of the present work was to determine whether the increase in monoamine oxidase activity previously found in hyperand hypothyroid rats, was due to thyroxin effects on enzymatic activity or to variations in enzyme synthesis. The rate of synthesis of monoamine oxidase in young hyperand hypothyroid rats has been studied by measuring the recovery of enzymatic activity 7 days after irreversib...