This paper deals with the recognition of spelled names over the telephone. It introduces an efficient way of handling the spelling grammar, that is the lexicon of the allowed spelled names. The proposed approach is based on a forward-backward algorithm. The constraints on the sequences of letters are derived from the lexicon and are used by the A* algorithm in the backward pass. This forward-ba...

Bovine myelin basic protein (MBP), charge isoform I (CI) was carboxylmethylated by the enzyme a-aspar ty l /Lisoaspartyl protein methyltransferase (EC. 2.1.1.77) and the carboxylmethylated protein was subjected to proteolysis by sequencing grade staphylococcal V 8 proteinase at pH 4.0 to identify its carboxylmethylated modified aspartate a n d / o r asparagine residues which are recognized by t...

The OleB protein of Streptomyces antibioticus, oleandomycin (OM) producer, constitutes an ATP-binding cassette transporter containing two nucleotide-binding domains and is involved in OM resistance and its secretion in this producer strain. We have characterized some properties of the first nucleotide-binding domain of OleB using an overexpressed fusion protein (MBP-OleB') between a maltose-bin...

RG13 is an engineered allosteric beta-lactamase (BLA) for which maltose is a positive effector. RG13 is a hybrid protein between TEM1 BLA and maltose-binding protein (MBP). Maltose binding to MBP is known to convert the open form of the protein to the closed form through conformational changes about the hinge region. We have constructed and genetically selected several variants of RG13 modified...

Assume Hin, Win and Cin denote the height, width and channel number of the input feature maps, respectively; Hout, Wout and Cout denote the height, width and channel number of the output feature maps, respectively; K is the side length of a square kernel. The forward pass of an And-layer, defined in Eq. (1), is of computational complexity O(KCinHoutWoutCout). For the backward pass, the complexi...

The NH2-terminal peptide of myelin basic protein (MBP) bound to the class II major histocompatibility complex (MHC) protein I-Au is an immunodominant epitope in experimental autoimmune encephalomyelitis, a murine model of multiple sclerosis. However, the MBP-I-Au complex is very unstable. To investigate this, we performed site-directed mutagenesis of the I-Au MHC protein and the MBP peptide. Bi...

The performance of deep neural networks is well-known to be sensitive to the setting of their hyperparameters. Recent advances in reverse-mode automatic differentiation allow for optimizing hyperparameters with gradients. The standard way of computing these gradients involves a forward and backward pass of computations. However, the backward pass usually needs to consume unaffordable memory to ...

It is well known that the classic Allen–Cahn equation satisfies maximum bound principle (MBP), is, absolute value of its solution uniformly bounded for all time by certain constant under suitable initial and boundary conditions. In this paper, we consider numerical solutions modified with a Lagrange multiplier nonlocal local effects, which not only shares same MBP as original but also conserves...

A DNA fragment encoding C-terminal BARc region (amino acids 128-416) of rat PICK1 (NP_445912 ) was inserted into a modified vector pMAL-s involving human rhinovirus 3C protease cleavage site to produce a recombinant plasmid, pMAL-s-barc. The construct can express the fusion protein, MBP-BARc in the soluble form in E.coli. To remove the MBP tag, MBP-BARc purified from amylose beads was digested ...

The preparation and characterization of an amperometric 2,4,6-trinitrotoluene (TNT) biosensor based on the surface immobilization of a maltose binding protein (MBP) nitroreductase (NR) fusion (MBP-NR) onto an electrode modified with an electropolymerized film of N-(3-pyrrol-1-ylpropyl)-4,4'-bipyridine (PPB) are described. The MBP domain of MBP-NR exhibits a high and specific affinity toward ele...