نتایج جستجو برای: mlst
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Despite more than 50 years of vaccination, Bordetella pertussis has remained endemic in The Netherlands, causing epidemic outbreaks every 3 to 5 years. Strain variation may play a role in the persistence of B. pertussis and was studied by sequencing 15 genes coding for surface proteins, including genes for all five components of acellular pertussis vaccines: pertussis toxin (Ptx), pertactin (Pr...
Multilocus sequence typing (MLST) has become the preferred method for genotyping many biological species, and it is especially useful for analyzing haploid eukaryotes. MLST is rigorous, reproducible, and informative, and MLST genotyping has been shown to identify major phylogenetic clades, molecular groups, or subpopulations of a species, as well as individual strains or clones. MLST molecular ...
Campylobacter jejuni is a common cause of the frequently reported food-borne diseases in developed and developing nations. This study describes the development of multiple-locus variable-number tandem-repeat (VNTR) analysis (MLVA) using capillary electrophoresis as a novel typing method for microbial source tracking and epidemiological investigation of C. jejuni. Among 36 tandem repeat loci det...
Typing strains within a microbial species on the basis of DNA sequences at multiple loci has greatly advanced study of the epidemiology and evolutionary phylogenetics of many bacterial (9, 40, 60, 72) and fungal (26, 68) pathogens. Multilocus sequence typing (MLST) schemes have now been published for five of the opportunistically pathogenic Candida species: C. albicans (6, 7), C. dubliniensis (...
24 Campylobacter jejuni is a common cause of the frequently reported foodborne diseases in 25 the developed and developing nations. This manuscript describes the development of multiple26 locus variable number tandem repeat analysis (MLVA) using capillary electrophoresis as a novel 27 typing method for microbial source tracking and epidemiological investigation of C. jejuni. 28 Among 36 tandem ...
A robust high-throughput multilocus sequence typing (MLST) scheme for Clostridium difficile was developed and validated using a diverse collection of 50 reference isolates representing 45 different PCR ribotypes and 102 isolates from recent clinical samples. A total of 49 PCR ribotypes were represented overall. All isolates were typed by MLST and yielded 40 sequence types (STs). A web-accessibl...
سابقه و هدف: اسینتوباکتر بامانی یک کوکوباسیل گرم منفی است که در طبیعت انتشار وسیعی داشته و به عنوان یکی از عوامل مهم عفونت های بیمارستانی محسوب می شود. مطالعه حاضر با هدف تعیین الگوی ژنتیکی جدایه های اسینتوباکتر بامانی جدا شده از عفونت های خون به روش تایپینگ ترادف یابی چند جایگاهی (MLST) انجام شد. مواد و روش ها: در این مطالعه مقط...
سابقه و هدف: اسینتوباکتر بامانی یک کوکوباسیل گرم منفی است که در طبیعت انتشار وسیعی داشته و به عنوان یکی از عوامل مهم عفونت های بیمارستانی محسوب می شود. مطالعه حاضر با هدف تعیین الگوی ژنتیکی جدایه های اسینتوباکتر بامانی جدا شده از عفونت های خون به روش تایپینگ ترادف یابی چند جایگاهی (MLST) انجام شد. مواد و روش ها: در این مطالعه مقط...
The Indigenous population of the Northern Territory of Australia (NT) suffers from a very high burden of Streptococcus pyogenes disease, including cardiac and renal sequelae. The aim of this study was to determine if S. pyogenes isolated from this population represent NT endemic strains, or conversely reflect strains with global distribution. emm sequence typing data were used to select 460 S. ...
This study used multilocus sequence typing (MLST) to investigate the prevalence of Helicobacter pylori ( H. ) mixed infections and involving unrelated strains; determined phylogeographic groups recovered from patients in Ningbo, China. A total 156 isolates were obtained a convenience sample 33 with culture-positive infection. MLST was classify 150 clinical 12 methodological control strains (6 6...
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