In this study, we investigated the effects of major royal jelly proteins (MRJPs) on estrogen, gut microbiome, and immunological responses in mice. Mice given 250 or 500 mg/kg, not 125 mg/kg MRJPs, enhanced proliferation splenocytes response to mitogens. The mesenteric lymphocytes activated by T-cell mitogens (ConA anti-CD3/CD28 antibodies) released high levels IL-2 but low IFN-γ IL-17A. release...

Abstract In mammalian cells, the decision to proliferate is thought be irreversibly made at restriction point of cell cycle 1,2 , when mitogen signalling engages a positive feedback loop between cyclin A2/cyclin-dependent kinase 2 (CDK2) and retinoblastoma protein 3–5 . Contrary this textbook model, here we show that actually fully reversible. Instead, find all cycling cells will exit in absenc...

objective(s): systemic candidiasis is an infection of candida albicans (c. albicans) causing disseminated disease and sepsis, invariably when host defenses are compromised. we investigated the histopathological changes as well as the lymphoproliferative responses and cytokine production of splenic cells after stimulation with concanavalin a (con a) and pokeweed mitogen (pwm) in mice with dissem...

In this study we examined the effect of mitogens and epidermal cells in inducing a Sézary cell morphology in normal peripheral blood lymphocytes. Peripheral blood mononuclear cells from six healthy volunteers were stimulated with the mitogens phytohemaglutinin and concanavalin A, and also cocultivated with human epidermal cell cultures. Incubation times with mitogens and epidermal cells were fo...

We attempted to generate continuous in vitro cultures from patients with mycosis fungoides and the Sezary syndrome (cutaneous T-cell lymphomas, CTCL). Using conventional culture techniques without mitogen stimulation. multiple attempts (32 specimens from 25 patients) failed to replicate T cells. but 6 B-lymphoblastoid cultures were established. Athymic nude mice injected by a variety of routes ...

In this study we examined the effect of mitogens and epidermal cells in inducing a S#{233}zary cell morphology in normal peripheral blood lymphocytes. Peripheral blood mononuclear cells from six healthy volunteers were stimulated with the mitogens phytohemaglutinin and concanavaIm A. and also cocultivated with human epidermal cell cultures. Incubation times with mitogens and epidermal cells wer...

The synthesis of induced nitric oxide (NO) is regulated by several cytokines, including growth factors produced following hepatic injury and inflammation. However, little information is available on the role of growth factors in regulating the inducible NO synthase in human hepatocytes. The capacity of hepatocellular mitogens (HGF, EGF, and TGF-alpha) to regulate the inducible NO synthase (iNOS...

This in vitro study was conducted to evaluate lymphocyte blastogenic and cytokine production by bovine peripheral blood mononuclear cells (PBMCs) stimulated with phytohemagglutinin (PHA), pokeweed mitogen (PWM) and concanavalin A (Con A) mitogens, by using tetrazolium salt and ELISA tests, respectively. The results presented that Interleukin-2 (IL-2), IL-4, IL-5, IL-10, IL-17 and IFN-γ producti...