This report demonstrates the ability of a microfluidic device to maintain human Graves' disease tissue enabling isolation and characterisation specific exosomes. (n = 7) non-Graves’ (Hashimoto's thyroiditis, n 3; follicular adenoma, 1) was incubated in for 6 days ± dexamethasone or methimazole effluent analysed size concentration extracellular vesicles (EV) using nanoparticle tracking analysis....

AIM To analyze expression of miRNA in human breast cancer cells, sensitive and resistant to cisplatin and doxorubicin, and to explore possible modification of drug sensitivity via treatment of cells with 5-azacytidine (5-aza), a demethylating agent. MATERIALS AND METHODS The study was performed on wild-type MCF-7 cell line (MCF-7/S) and its two sublines MCF-7/Dox and MCF-7/DDP resistant to do...

objective: micrornas (mirnas) are single-stranded small rnas 18-25 nucleotides in length that regulate gene expression through translational inhibition and mrna cleavage. aberrant expression of mirnas contribute to several diseases. this has increased interest in profiling the expressions of these molecules. real-time quantitative pcr (rq-pcr) is a sensitive, quantitative technique for gene exp...

Altered expression of miRNAs may contribute to multidrug resistance (MDR) in human cancers. This study investigated the association between miRNAs and MDR in five different drug-resistant hepatocellular carcinoma (HCC) cell sublines. The HCC Huh-7 cell line was treated with adramycin (ADM), cisplatin (DDP), carboplatin (CBP), mitomycin C (MMC) or vincristine (VCR) at increasing concentrations t...

The embryonic stem cell cycle (ESCC) and let-7 families of miRNAs function antagonistically in the switch between mouse embryonic stem cell self-renewal and somatic differentiation. Here, we report that the human ESCC miRNA miR-372 and let-7 act antagonistically in germline differentiation from human embryonic stem cells (hESCs) and human induced pluripotent stem cells (iPSCs). hESC and iPSC-de...

Major RNA products of a microRNA (miRNA) gene--the long primary transcript (pri-miRNA), the ∼70-nucleotide (nt) precursor miRNA (pre-miRNA), and the ∼21-nt mature miRNA--all contain the same sequence required for target gene recognition. Thus, it is intrinsically difficult to discern the contribution of individual RNA species or to rule out a function of miRNA precursor species in target repres...

BACKGROUND Mineral Pitch (MP) is a dark brown coloured humic matter originating from high altitude rocks. It is an Ayurvedic medicinal food, commonly used by the people of the Himalayan regions of Nepal and India for various body ailments. METHODS The Huh-7 cells were treated with different concentrations of MP for 24 h, and both apoptosis and proliferation was determined by the TUNEL and MTT...