In this paper, the effect of microwave-assisted heating (60, 80, 100 and 120 °C) on lignin separation from ball-milled hybrid poplar wood in dimethyl sulfoxide and lithium chloride (DMSO/LiCl) system was examined. The isolated lignin fractions were characterized by HPAEC (high-performance anion exchange chromatography), FT-IR, C NMR and 2D-HSQC NMR techniques. The results showed that extraction...

Enzymatic hydrolysis of cellulose in plant and wood cell walls is expected to be affected by its chemical composition as well as structural and morphological features. In the present study, different crystallinity cellulose samples and varying size fractionated loblolly pine wood cell wall particles were hydrolyzed using a mixture of Celluclast and Novozyme 188. In pure cellulose samples of Wha...

Aspen wood was subjected to autohydrolysis as a pre-treatment to characterize the structural changes occurring in lignin fractions during the pre-treatment process. Milled wood lignin (MWL) was isolated from both the native aspen wood and hydrolyzed wood chips, and its structural features were characterized by Fourier transform infrared (FT-IR), quantitative 13C, two-dimensional heteronuclear s...

We have studied the structural modifications of lignins from sisal and flax during their soda-anthraquinone (AQ) pulping and subsequent totally chlorine-free (TCF) and elementary chlorine-free (ECF) bleaching. For this purpose, residual lignins were isolated from pulps, analyzed by Py-GC/MS, 2D-NMR, and GPC, and their characteristics were compared to the “milled-wood” lignin of the raw material...

For research and development of plants suitable for forage and biofuel production, the development of a highthroughput system of lignin determination, especially for herbaceous species, is important. However, currently available methods for lignin determination are not suitable for high-throughput analysis of herbaceous samples. In this paper, we describe a straightforward, high-throughput meth...

Milled wood lignin (MWL), cellulolytic enzyme lignin (CEL), and enzymatic mild acidolysis lignin (EMAL) were isolated from different wood species and characterized by various techniques. The EMAL protocol offered gravimetric lignin yields 2-5 times greater than those of the corresponding MWL and CEL. The purities of the EMALs were 3.75-10.6% higher than those of their corresponding CELs, depend...

Depolymerization of lignin in wood with hydrogen iodide in a nonpolar solvent is a selective, high-yield reaction that releases a diiodide of potential synthetic value into the solution. Finely milled wood (Douglas-fir, spruce, aspen, and sugarcane), was suspended in CDCl3 and treated with dry hydrogen iodide in a NMR tube. The yields and composition of the chloroform-soluble monomeric lignin d...

Lignin from furfural residue was extracted with alkaline hydrogen peroxide at different temperatures and times. The structural features of lignins were characterized by elemental analysis, GPC, FT-IR, H NMR, C NMR and Py-GC/MS. Results showed that the yield of lignin increased with the increase of reaction time and temperature. The maximum lignin yield of 41.4% (corresponding to the original li...

BACKGROUND A key focus in sustainable biofuel research is to develop cost-effective and energy-saving approaches to increase saccharification of lignocellulosic biomass. Numerous efforts have been made to identify critical issues in cellulose hydrolysis. Aerobic fungal species are an integral part of the carbon cycle, equip the hydrolytic enzyme consortium, and provide a gateway for understandi...