The present study was carried out to assess the efficacy of microtiter method of direct antiglobulin test in diagnosis of Immune Mediated Haemolytic Anaemia in Dogs. Two hundred and fifty eight anaemic dogs with pale/icteric mucous membrane were used for the present study. Initial screening of these dogs was done with saline agglutination test. Forty seven dogs were found positive for saline ag...

Smooth, rough, and neutral forms of lipopolysaccharide (LPS) from Pseudomonas aeruginosa were used to assess the appropriate conditions for effective enzyme-linked immunosorbent assay (ELISA) of LPS. Each of these forms of well-defined LPS was tested for the efficiency of antigen coating by various methods as well as to identify an appropriate type of microtiter plate to use. For smooth LPS, th...

DISEASE DIAGNOSIS FROM IMMUNOASSAYS WITH PLATE TO PLATE VARIABILITY Oliver Entine Dylan Small The standard methods of diagnosing disease based on antibody microtiter plates are quite crude. Few methods create a rigorous underlying model for the antibody levels of populations consisting of a mixture of positive and negative subjects, and fewer make full use of the entirety of the available data ...

A quantitative microtiter method for determining the degree of complement resistance or sensitivity of microorganisms is described. The microtiter method is compared with a quantitative automated system and the standard plate-count technique. Data were accumulated from 30 avian Escherichia coli isolates incubated at 35 C with either chicken plasma or heat-inactivated chicken plasma. Analysis of...

We have developed a fluorogenic NAD(P)(+) detection method using 2-acetylbenzofuran. The reaction of NAD(P)(+) with 2-acetylbenzofuran produced a fluorescent product, allowing the highly-sensitive and quick detection of NAD(P)(+). This method was successfully applied to the detection of P450 substrates in the microtiter-plate format.

BACKGROUND High-throughput methods are widely-used for strain screening effectively resulting in binary information regarding high or low productivity. Nevertheless achieving quantitative and scalable parameters for fast bioprocess development is much more challenging, especially for heterologous protein production. Here, the nature of the foreign protein makes it impossible to predict the, e.g...

We have developed a simple gene quantification system using the competitive polymerase chain reaction (CPCR) followed by microtiter format analysis. CPCR is carried out using a mutant competitor with the same size as the target DNA product, and a minimal base exchange to insure the same amplification kinetics. One primer is aminated at the 5' end to produce PCR products that are captured onto c...

BACKGROUND Biofilms are communities of bacteria attached to the surfaces in an extracellular polymeric matrix which are associated with many chronic infections in humans. Acinetobacter spp. are emerging as a major cause of nosocomial infections and Acinetobacter baumannii is the predominant species associated with this kind of infections. OBJECTIVES In the present study, the potential of biof...