Microsatellites are short sequence repeats that make up the genomes of eukaryotes and prokaryotes. They great importance as DNA markers for studies in several fields genetics. In present review, we searched published five years period 2017 to 2021 regarding use microsatellites with forest tree species from Brazilian biomes, order examine these resources conservation. We scientific papers journa...

Understanding how microsatellites are distributed in eukaryotic genomes is important to clarify the differential abundance of these repeats under an evolutionary scenario. We have concatenated data from 3165 DNA sequences of 326 Bivalvia species to search for taxonomic patterns of microsatellite distribution in genomic regions of markedly different functionality. Some microsatellite motifs in b...

AIMS To develop an assay which would determine the parentage of hydatidiform molar pregnancies. METHODS DNA was extracted from formalin fixed, paraffin wax embedded tissue from hydatidiform molar pregnancies and spontaneous abortions after separation of chorionic villi and decidua. PCR amplification of dinucleotide repeat sequences ("microsatellites") was performed using three different prime...

for identification of individuals and parentage control performed by cattle breeders in the czech republic, a novel finnish bovine genotypes™ panel 3.1was amplified by means of one multiplex polymerase chain reaction. bovine panel encompasses all the 12 str loci recommended by the international society for animal genetics (isag) for routine use in parentage testing and identification, including...

Microsatellites are ubiquitously present in eukaryotic genomes and are implicated as positive factors in evolution. At the nucleotide level, microsatellites undergo slippage events that alter allele length and base changes that interrupt the repetitive tract. We examined DNA polymerase errors within a [T](11) microsatellite using an in vitro assay that preferentially detects mutations other tha...

We examined the stability of microsatellites of different repeat unit lengths in Saccharomyces cerevisiae strains deficient in DNA mismatch repair. The msh2 and msh3 mutations destabilized microsatellites with repeat units of 1, 2, 4, 5, and 8 bp; a poly(G) tract of 18 bp was destabilized several thousand-fold by the msh2 mutation and about 100-fold by msh3. The msh6 mutations destabilized micr...

The advent of DNA technology over the recent years has led to the development of molecular markers that are highly precise, convenient and cost effective for detection of polymorphism among individuals and also for individual identification. These molecular marker techniques viz., Restriction fragment length polymorphism, Random amplified polymorphic DNA, Amplified fragment length polymorphism,...

Thirteen nuclear-encoded microsatellites from a genomic DNA library of Serra Spanish mackerel, Scomberomorus brasiliensis, were isolated and characterized. The microsatellites include 10 perfect repeats (eight tetranucleotide and two dinucleotide) and three imperfect repeats (two tetranucleotide and one dinucleotide). An additional five microsatellites, isolated originally from two congeneric s...

Ten microsatellites were isolated from a genomic DNA library generated from guppy (Poecilia reticulata; Poecilidae) enriched for CA-repeats. All of the 10 microsatellites were polymorphic in guppy with an average allele number of 4.9/locus ranging from 2 to 14. All 10-primer pairs amplified specific products in green swordtail (Xiphophorus helleri) and 9 of the 10 microsatellites displayed poly...