Production of tanshinones (tanshinone I and IIA) was determined in roots of Salvia przewalskii micropropagated plants. It was found that the total tanshinone content (tashinone I and tashinone IIA) was dependent on the age of the analyzed plants. The roots of 2-year-old in vitro regenerated plants at flowering stage produced highest tanshinone levels (3.8 mg/g dry weight of tanshinone I and 7.6...

An experiment was conducted to evaluate the tolerance of micropropagated and mycorrhized alpinia plants to the parasite Meloidogyne arenaria. The experimental design was completely randomized with a factorial arrangement of four inoculation treatments with arbuscular mycorrhizal fungi (AMF) (Gigaspora albida, Claroideoglomus etunicatum, Acaulospora longula, and a non-inoculated control) in the ...

Higher plants are ubiquitously colonized with fungal endophytes that often lack readily detectable structures. This study examines the diverse endophyte population within a single line of micropropagated Bouteloua eriopoda (Torr.) Torr., using microscopy and comparison of internal spacer (ITS) gene sequences obtained from both plant and isolated fungal tissues. Microscopy revealed fungal hyphae...

Micropropagated plants are usually cultured in in vitro environments, with a high relative humidity and low light intensity, taking nutrients and energy from the culture medium. During acclimatization, physiological and structural changes allow micropropagated plants to adapt to the new environmental conditions, mainly to low relative humidity and high light intensity. As a result, plants becom...

Micropropagated plantlets derived from three different grape rootstock genotypes namely, Dogridge (Vitis champini), SO4 (V. berlandieri×V. rupestris) and ARI-H-144 (V. vinifera×V. labrusca) were subjected to randomly amplified polymorphic DNA (RAPD) and inter simple sequence repeats (ISSR) analyses in order to evaluate their genetic stability and/or detect likely existing variations among in vi...

The present study was attempted to enhance the anticancer activities in in vitro grown medicinal plant, Vernonia divergens, generated under the stress of a fungus, Piriformospora indica and to evaluate the effect of such biotization against EAC cell lines using standard MTT assay. The effect of adenine sulphate in addition to the phytohormones was assessed for micropropagation of V. divergens f...

Genetic variation due to somaclonal variation in micropropagated plants is a beneficial phenomenon for crop improvement. Genetic integrity of the plants derived through micropropagation becomes crucial if genetic transformation studies have to be carried out. Somaclonal variation in tissue culture is a common phenomenon which makes it mandatory to check for genetic stability of plants. Hypocoty...

Pittosporum eriocarpum Royle, a medicinally important taxon, is endemic to Uttarakhand region of Himalaya. It has become endangered due to over-collection and the loss of habitats. As raising plants through seeds in this plant is problematic, a reliable protocol for micropropagation using nodal explants has been developed. High shoot regeneration (95%) occurred in MS medium augmented with BA 0....

Micropropagation of woody plants and fruit crops constitutes a major success in the commercial application of in vitro cultures. An important aspect to be considered when deriving perennial plants from micropropagation is the maintenance of genetic integrity with regard to the mother plant. In this regard, somaclonal variation has been reported at different levels (morphological, cytological, c...

"Influence of Arbuscular Mycorrhizal Fungi on the Growth and Development Micropropagated Rubus fruticosus ‘P45’ Plants during Acclimatization" published Aug 2023 by American Society for Horticultural Science.