نتایج جستجو برای: microplate
تعداد نتایج: 2640 فیلتر نتایج به سال:
We adapted the method of McCaman and Robins for fluorometry of phenylalanine to a microplate assay for routine phenylketonuria screening. Sensitivity is 15 mumol/L for the plasma assay and 30 mumol/L for the dried blood-spot assay, with CV less than 10% for both assays. Results for human plasma by microplate assay correlated well (r = 0.99) with results of amino acid analyzer determination of p...
The microplate format has become increasingly popular in biological experimentation due to its small size, easy sample handling, increased throughput, decreased sample preparation, and small sample volume. The need to perform increasing numbers of assays has sparked interest in high throughput, microplate compatible radioisotope counters. Many of these biological experiments are performed on gl...
The microplate format has become a standard for many biological assays such as high throughput screening for drug discovery and radioimmunoassays. Productivity with these high volume procedures has been limited by the amount of sample handling required and low sample counting throughput. Now, however, microplate assays can be efficiently counted in microplates with the Packard TopCount Micropla...
This paper describes a robust and rapid procedure for measuring chlorophyll using a microplate reader. The procedure enables direct translation of any published equation developed on a 1-cm pathlength spectrophotometer to any format microplate with any sample volume. Chlorophylls were extracted with methanol because it is compatible with cheap polystyrene plates; however, the principles apply t...
In this paper, two-variable refined plate theory and strain gradient (SGT) are adopted termed (TV-SGT) for buckling analysis of moderately thick microplate. The TV-SGT embraces both shear micro-effects simultaneously and, therefore, does not need the correction factor. Notably, governing equations new boundary conditions derived using variational approach. Moreover, generalized complicated prob...
The aim of the present work was to estimate the possibility of improving the objectiveness of complement fixation test (CFT) in the diagnosis of dourine by using an ELISA microplate reader. The investigations were carried out on control positive and negative sera and 16 sera obtained for a reference examination. Six modifications of CFT performing and reading methods were compared (tube, microp...
An attempt was made to demonstrate the possibility of performing LanthaScreen™ TR-FRET based nuclear receptor coactivator recruitment assay using PHERAstar, a multi-detection HTS microplate reader. LanthaScreen™ nuclear receptor coactivator recruitment assay (M/s Invitrogen corporation, USA) was performed using PPAR-gamma receptor preparation in the agonist mode. TR-FRET measurements were done ...
Commonly, intra-continental wrenching is associated with a high degree of crustal faulting and fragmentation. The resulting continental blocks can undergo vertical-axis rotations, which in turn lead to the generation intricate fault networks within along their boundary regions. Investigations into these structural complexities support understanding when how rotate, what position was prior trans...
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